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Immunohistochemistry (IHC)-Paraffin (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using MBS7129079(HSPE1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

Rabbit HSPE1 Polyclonal Antibody | anti-HSPE1 antibody

HSPE1 Antibody

Gene Names
HSPE1; EPF; CPN10; GROES; HSP10
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
HSPE1; Polyclonal Antibody; HSPE1 Antibody; heat shock 10kDa protein 1; anti-HSPE1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
Liquid; In Phosphate Buffered Saline, pH 7.4, 0.05% NaN3, and 40% Glycerol
Applicable Applications for anti-HSPE1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
ELISA: 1:1000-1:2000
WB: 1:200-1:1000
IHC: 1:50-1:200
Immunogen
Synthetic peptide of human HSPE1
Immunogen Species
Human
Conjugate
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)-Paraffin

(The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using MBS7129079(HSPE1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC)-Paraffin (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using MBS7129079(HSPE1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC)-Paraffin

(The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using MBS7129079(HSPE1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC)-Paraffin (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using MBS7129079(HSPE1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

SDS-Page

(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: HepG2 cells, Primary antibody: MBS7129079(HSPE1 Antibody) at dilution 1/750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes)

SDS-Page (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: HepG2 cells, Primary antibody: MBS7129079(HSPE1 Antibody) at dilution 1/750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes)
Product Categories/Family for anti-HSPE1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,932 Da
NCBI Official Full Name
10 kDa heat shock protein, mitochondrial
NCBI Official Synonym Full Names
heat shock 10kDa protein 1 (chaperonin 10)
NCBI Official Symbol
HSPE1
NCBI Official Synonym Symbols
EPF; CPN10; GROES; HSP10
NCBI Protein Information
10 kDa heat shock protein, mitochondrial; chaperonin 10; 10 kDa chaperonin; early-pregnancy factor; heat shock 10kD protein 1 (chaperonin 10)
UniProt Protein Name
10 kDa heat shock protein, mitochondrial
UniProt Gene Name
HSPE1
UniProt Synonym Gene Names
Hsp10; CPN10; EPF
UniProt Entry Name
CH10_HUMAN

NCBI Description

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]

Uniprot Description

HSPE1: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Belongs to the GroES chaperonin family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: membrane; mitochondrion; mitochondrial matrix

Molecular Function: protein binding; chaperone binding; unfolded protein binding; ATP binding

Biological Process: caspase activation; osteoblast differentiation; protein folding; response to unfolded protein

Research Articles on HSPE1

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Product Notes

The HSPE1 hspe1 (Catalog #AAA7129079) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPE1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSPE1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ELISA: 1:1000-1:2000 WB: 1:200-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the HSPE1 hspe1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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