Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain reacted with HSPC142 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Human HSPC142 Polyclonal Antibody | anti-HSPC142 antibody
HSPC142 (BRCA1-A Complex Subunit MERIT40, Chromosome 19 Open Reading Frame 62, C19orf62, FLJ20571, Mediator of RAP80 Interactions and Targeting Subunit of 40kD, MERIT40, New Component of the BRCA1-A Complex, NBA1) (AP)
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain reacted with HSPC142 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain reacted with HSPC142 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB)
(Western blot analysis of HSPC142 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). HSPC142 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of HSPC142 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). HSPC142 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MDA-MB468 cells using HSPC142 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MDA-MB468 cells using HSPC142 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
NBA1: a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36 component. The VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes. Two alternatively spliced human isoforms have been reported.
Protein type: DNA repair, damage
Chromosomal Location of Human Ortholog: 19p13.11
Cellular Component: cytoplasm; nucleoplasm; nucleus
Molecular Function: protein binding
Biological Process: double-strand break repair; double-strand break repair via nonhomologous end joining; G2/M transition DNA damage checkpoint; positive regulation of DNA repair; response to ionizing radiation
Research Articles on HSPC142
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Product Notes
The HSPC142 babam1 (Catalog #AAA6481195) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPC142 (BRCA1-A Complex Subunit MERIT40, Chromosome 19 Open Reading Frame 62, C19orf62, FLJ20571, Mediator of RAP80 Interactions and Targeting Subunit of 40kD, MERIT40, New Component of the BRCA1-A Complex, NBA1) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSPC142 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HSPC142 babam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPC142, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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