Western Blot (WB)
(Western blot analysis of Rat tissue mix showing detection of HSPB2 protein using Rabbit Anti-HSPB2 Polyclonal Antibody (SPC-194). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSPB2 Polyclonal Antibody (SPC-194) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Rabbit HSPB2 (MKBP) Polyclonal Antibody | anti-HSPB2 antibody
HSPB2 (MKBP) Antibody: ATTO 390
Gene Names
HSPB2; MKBP; HSP27; Hs.78846; LOH11CR1K
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Synonyms
HSPB2 (MKBP); Polyclonal Antibody; HSPB2 (MKBP) Antibody: ATTO 390; DMPK-binding protein; heat shock 27kDa protein 2; Hsp27; HspB2; MKBP; MGC133245; anti-HSPB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
182
Applicable Applications for anti-HSPB2 antibody
Western Blot (WB)
Application Notes
1:1000 (WB)
Conjugate
ATTO 390
Storage Buffer
PBS, 50% glycerol, 0.02% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western blot analysis of Rat tissue mix showing detection of HSPB2 protein using Rabbit Anti-HSPB2 Polyclonal Antibody (SPC-194). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSPB2 Polyclonal Antibody (SPC-194) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Western Blot (WB)
(Western blot analysis of Rat tissue mix showing detection of HSPB2 protein using Rabbit Anti-HSPB2 Polyclonal Antibody (SPC-194). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSPB2 Polyclonal Antibody (SPC-194) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Related Product Information for anti-HSPB2 antibody
Background Info: Detects ~27kDa
Scientific Background: HSPB2, also known as MKBP is the most divergent member of the sHSP family with only 30% sequence identity to all other mammalian sHSPS (1). MKBP is known to associate specifically with myotonic dystrophy protein kinase (DMPK) in skeletal muscle. MKBP enhances the kinase activity of DMPK and protects it from heat-induced activation. MKBP also shows a unique nature compared to other sHSps, in that the expression of MKBP is not induced by heat shock (2). In unstressed skeletal muscle, MKBP forms large oligomeric complexes with HSPB3 in the cytosol which are localized on mitochondria and the neuromuscular junction (1, 3). During stress, these complexes dissolve and MKBP's localization to mitochondria increases, leading to increased cell survival. Pinz et al. tried to find a distinct role for MKBP in terms of cardiac mechanics and finds that it is required for normal systolic performance and normal cardiac energetic (1). HSPB2 has also been found to be expressed in several cancer cell lines, including human breast cancer, suggesting that MKBP may be an important factor in tumor transformation and metastasis (4).
Scientific Background: HSPB2, also known as MKBP is the most divergent member of the sHSP family with only 30% sequence identity to all other mammalian sHSPS (1). MKBP is known to associate specifically with myotonic dystrophy protein kinase (DMPK) in skeletal muscle. MKBP enhances the kinase activity of DMPK and protects it from heat-induced activation. MKBP also shows a unique nature compared to other sHSps, in that the expression of MKBP is not induced by heat shock (2). In unstressed skeletal muscle, MKBP forms large oligomeric complexes with HSPB3 in the cytosol which are localized on mitochondria and the neuromuscular junction (1, 3). During stress, these complexes dissolve and MKBP's localization to mitochondria increases, leading to increased cell survival. Pinz et al. tried to find a distinct role for MKBP in terms of cardiac mechanics and finds that it is required for normal systolic performance and normal cardiac energetic (1). HSPB2 has also been found to be expressed in several cancer cell lines, including human breast cancer, suggesting that MKBP may be an important factor in tumor transformation and metastasis (4).
Product Categories/Family for anti-HSPB2 antibody
References
1. Pinz I., et al. (2008) FASEB J. 22: 84-92. 2. Suzuki A., et al. (1998) J Cell Bio. 140(5): 1113-1124. 3. Sugiyama Y., et al. (2000) J Biol Chem. 275: 1095-1104. 4. Oshita S.E., et al. (2010) Breast Cancer Res Treat. Epub: 20087649.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,233 Da
NCBI Official Full Name
heat shock protein beta-2
NCBI Official Synonym Full Names
heat shock 27kDa protein 2
NCBI Official Symbol
HSPB2
NCBI Official Synonym Symbols
MKBP; HSP27; Hs.78846; LOH11CR1K
NCBI Protein Information
heat shock protein beta-2; DMPK-binding protein; heat-shock protein beta-2
UniProt Protein Name
Heat shock protein beta-2
Protein Family
UniProt Gene Name
HSPB2
UniProt Synonym Gene Names
HspB2; MKBP
UniProt Entry Name
HSPB2_HUMAN
NCBI Description
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The protein is expressed preferentially in the heart and skeletal muscle. This protein regulates Myotonic Dystrophy Protein Kinase, which plays an important role in maintenance of muscle structure and function. [provided by RefSeq, Dec 2012]
Uniprot Description
HSPB2: May regulate the kinase DMPK. Belongs to the small heat shock protein (HSP20) family.
Protein type: Heat shock protein; Chaperone
Chromosomal Location of Human Ortholog: 11q22-q23
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: protein binding; enzyme activator activity
Biological Process: positive regulation of catalytic activity; response to unfolded protein
Research Articles on HSPB2
Similar Products
Product Notes
The HSPB2 hspb2 (Catalog #AAA806237) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPB2 (MKBP) Antibody: ATTO 390 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSPB2 (MKBP) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). 1:1000 (WB). Researchers should empirically determine the suitability of the HSPB2 hspb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPB2 (MKBP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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