Rabbit anti-Human HSPA9 Polyclonal Antibody | anti-HSPA9 antibody

HSPA9, ID (HSPA9, GRP75, HSPA9B, Stress-70 protein, mitochondrial, 75kD glucose-regulated protein, Heat shock 70kD protein 9, Mortalin, Peptide-binding protein 74) (PE)

Cat. Num. AAA6308704

• Gene Names: HSPA9; CSA; MOT; MOT2; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HSPA9; Polyclonal Antibody; ID (HSPA9; GRP75; HSPA9B; Stress-70 protein; mitochondrial; 75kD glucose-regulated protein; Heat shock 70kD protein 9; Mortalin; Peptide-binding protein 74) (PE); Peptide-binding protein 74; anti-HSPA9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HSPA9 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HSPA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-309 amino acids from the Central region of human HSPA9.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HSPA9 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HSPA9 antibody detected the HSPA9 protein (arrow).)

Related Product Information for anti-HSPA9 antibody

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.

Product Categories/Family for anti-HSPA9 antibody

Antibodies; Heat Shock Proteins

References

Li, Y., et al. Environ. Health Perspect. 118(7):936-942(2010). Luo, W.I., et al. Protein Expr. Purif. 72(1):75-81(2010). Goswami, A.V., et al. J. Biol. Chem. 285(25):19472-19482(2010). Iosefson, O., et al. FEBS Lett. 584(6):1080-1084(2010). Rikova, K., et al. Cell 131(6):1190-1203(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 24234688
• NCBI GeneID: 3313
• NCBI Accession #: NP_004125.3
• NCBI GenBank Nucleotide #: NM_004134.6
• UniProt Accession #: P38646; P30036; P31932; Q1HB43; Q53H23; Q6GU03; Q9BWB7; Q9UC56; B2RCM1
• Molecular Weight: 679
• NCBI Official Full Name: stress-70 protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock 70kDa protein 9 (mortalin)
• NCBI Official Symbol: HSPA9
• NCBI Official Synonym Symbols: CSA; MOT; MOT2; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75
• NCBI Protein Information: stress-70 protein, mitochondrial; mortalin-2; p66-mortalin; mortalin, perinuclear; heat shock 70kD protein 9B; peptide-binding protein 74; 75 kDa glucose-regulated protein
• UniProt Protein Name: Stress-70 protein, mitochondrial
• Protein Family: Stress-70 protein
• UniProt Gene Name: HSPA9
• UniProt Synonym Gene Names: GRP75; HSPA9B; mt-HSP70; GRP-75; MOT; PBP74
• UniProt Entry Name: GRP75_HUMAN

Uniprot Description

HSPA9B: Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. Interacts with FXN. Interacts with HSCB. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2. Belongs to the heat shock protein 70 family.

Protein type: Nucleolus; Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: focal adhesion; mitochondrion; cytoplasm; nucleolus

Molecular Function: protein binding; ubiquitin protein ligase binding; unfolded protein binding; ATP binding

Biological Process: cellular protein metabolic process; protein folding; protein export from nucleus; protein targeting to mitochondrion; negative regulation of apoptosis