Rabbit anti-Human HSPA6 / HSP70B' Polyclonal Antibody | anti-HSPA6 / HSP70B antibody

Anti-HSPA6 / HSP70B' Antibody (aa377-628) IHC-plus

Cat. Num. AAA2400255

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: HSPA6 / HSP70B'; Polyclonal Antibody; Anti-HSPA6 / HSP70B' Antibody (aa377-628) IHC-plus; Rabbit Polyclonal (IgG) to Human HSPA6 / HSP70B' ; Human HSPA6 / HSP70B' Heat shock 70 kd protein b; Heat shock 70 kDa protein 6; Heat shock 70 kDa protein B; Heat shock 70 kd protein 7; anti-HSPA6 / HSP70B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human HSPA6
• Purity/Purification: Immunoaffinity purified
• Form/Format: 0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 643

• Applicable Applications for anti-HSPA6 / HSP70B antibody: Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC (1:100 - 1:1000); IHC-P (7.5 ug/ml); WB (1:500 - 1:3000)
• Immunogen: HSPA6 / HSP70B' antibody was raised against recombinant fragment corresponding to a region within amino acids 377 and 628 of HSPA6 (SwissProt P17066).
• Immunogen Description: Recombinant fragment corresponding to a region within amino acids 377 and 628 of HSPA6 (SwissProt P17066).
• Immunogen Type: Recombinant protein
• Antigen Modification: aa377-628
• Target Species: Human
• Preparation and Storage: Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-HSPA6 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)

Immunocytochemistry (ICC)

(Immunofluorescence of paraformaldehyde-fixed A431 using HSPA6 antibody at 1:200 dilution.)

Western Blot (WB)

(Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. HSPA6 antibody diluted at 1:1000)

Related Product Information for anti-HSPA6 / HSP70B antibody

HSPA6 Antibody, Heat shock 70 kd protein b Antibody, Heat shock 70 kDa protein 6 Antibody, Heat shock 70 kDa protein B Antibody, Heat shock 70 kd protein 7 Antibody, HSP70B Antibody Description: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 34419635
• NCBI GeneID: 3310
• NCBI Accession #: NP_002146.2
• NCBI GenBank Nucleotide #: NM_002155.4
• UniProt Accession #: P17066; Q1HBA8; Q8IYK7; Q9BT95
• Molecular Weight: 71,028 Da
• NCBI Official Full Name: heat shock 70 kDa protein 6
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 6
• NCBI Official Symbol: HSPA6
• NCBI Protein Information: heat shock 70 kDa protein 6
• UniProt Protein Name: Heat shock 70 kDa protein 6
• UniProt Gene Name: HSPA6
• UniProt Synonym Gene Names: HSP70B'
• UniProt Entry Name: HSP76_HUMAN

Uniprot Description

HSPA6: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Belongs to the heat shock protein 70 family.

Protein type: Heat shock protein

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: centriole; cytoplasm; cytosol; signalosome

Molecular Function: ATP binding; ATPase activity, coupled; enzyme binding; heat shock protein binding; protein binding; unfolded protein binding

Biological Process: protein refolding; response to unfolded protein