Rabbit HSPA5 / GRP78 / BIP Polyclonal Antibody | anti-HSPA5 antibody

Rabbit Polyclonal to Rat HSPA5 / GRP78 / BIP

Cat. Num. AAA248842

• Gene Names: HSPA5; BIP; MIF2; GRP78; HEL-S-89n
• Reactivity: Bovine, Xenopus, Fungus, Hamster, Human, Monkey, Mouse, Rabbit, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Unpurified serum
• Synonyms: HSPA5 / GRP78 / BIP; Polyclonal Antibody; Rabbit Polyclonal to Rat HSPA5 / GRP78 / BIP; Anti-HSPA5 / GRP78 / BIP Antibody (aa645-654) IHC-plus; HSPA5; BIP; Heat shock 70 kDa protein 5; MIF2; GRP-78; GRP78; Rat HSPA5; anti-HSPA5 antibody

• Host: Rabbit
• Reactivity: Bovine, Xenopus, Fungus, Hamster, Human, Monkey, Mouse, Rabbit, Rat
• Clonality: Polyclonal
• Specificity: Detects a protein with a mass of ~78 kD corresponding to Grp78.
• Purity/Purification: Unpurified serum
• Form/Format: Antiserum
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 654

• Applicable Applications for anti-HSPA5 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (1:100), WB (1:2000)
• Target Species: Rat
• Immunogen Description: Rat GRP78 (Bip) synthetic peptide conjugated to KLH.
• Immunogen Type: Synthetic peptide - KLH conjugated
• Immunogen: HSPA5 / GRP78 / BIP antibody was raised against rat GRP78 (Bip) synthetic peptide conjugated to KLH.
• Antigen Modification: aa645-654
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-HSPA5 / GRP78 / BIP antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-HSPA5 / GRP78 / BIP antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(Grp78, Rat tissues.)

Western Blot (WB)

(Grp78 (Bip), Rat tissue Mix, polyclonal.)

NCBI and Uniprot Product Information

• NCBI GI #: 16507237
• NCBI GeneID: 3309
• NCBI Accession #: NP_005338.1
• NCBI GenBank Nucleotide #: NM_005347.4
• UniProt Accession #: P11021; Q2EF78; Q9NPF1; Q9UK02; B0QZ61
• Molecular Weight: 72,333 Da
• NCBI Official Full Name: 78 kDa glucose-regulated protein
• NCBI Official Synonym Full Names: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• NCBI Official Symbol: HSPA5
• NCBI Official Synonym Symbols: BIP; MIF2; GRP78; HEL-S-89n
• NCBI Protein Information: 78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; epididymis secretory sperm binding protein Li 89n; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
• UniProt Protein Name: 78 kDa glucose-regulated protein
• UniProt Gene Name: HSPA5
• UniProt Synonym Gene Names: GRP78; GRP-78; BiP
• UniProt Entry Name: GRP78_HUMAN

NCBI Description

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

Uniprot Description

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: signalosome; endoplasmic reticulum membrane; cell surface; focal adhesion; smooth endoplasmic reticulum; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment; membrane; plasma membrane; melanosome; integral to endoplasmic reticulum membrane; midbody; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; chaperone binding; unfolded protein binding; ATPase activity; ribosome binding; misfolded protein binding; calcium ion binding; glycoprotein binding; ATP binding

Biological Process: platelet activation; ER-associated protein catabolic process; cerebellum structural organization; unfolded protein response; cerebellar Purkinje cell layer development; cellular response to glucose starvation; platelet degranulation; cellular protein metabolic process; substantia nigra development; unfolded protein response, activation of signaling protein activity; positive regulation of protein ubiquitination; blood coagulation; negative regulation of transforming growth factor beta receptor signaling pathway; ER overload response; positive regulation of cell migration; negative regulation of apoptosis