Rabbit anti-Human HSPA4L Polyclonal Antibody | anti-HSPA4L antibody

HSPA4L, CT (Heat shock 70-related protein APG-1, Osmotic stress protein 94, Heat shock 70kD protein 4L, HSPA4L, APG1, OSP94) (MaxLight 550)

Cat. Num. AAA6479681

• Gene Names: HSPA4L; APG-1; HSPH3; Osp94
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HSPA4L; Polyclonal Antibody; CT (Heat shock 70-related protein APG-1; Osmotic stress protein 94; Heat shock 70kD protein 4L; APG1; OSP94) (MaxLight 550); anti-HSPA4L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HSPA4L.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-HSPA4L antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-1:50; WB: 1:1000; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 776-803 of the C-terminal region of human HSPA4L Accession NP_055093.2, O95757.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HSPA4L Antibody (C-term) in Hela cell line lysates (35ug/lane). HSPA4L (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with MBS629968 which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Flow Cytometry (FC/FACS)

(HSPA4L Antibody (C-term) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-HSPA4L antibody

Antibodies; Heat Shock Proteins

References

1. Nonoguchi, K., et.al., Int. J. Urol. 8(6), 308-314 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 31541941
• NCBI GeneID: 22824
• NCBI Accession #: NP_055093.2
• NCBI GenBank Nucleotide #: NM_014278.3
• UniProt Accession #: O95757; Q4W5M5; Q8IWA2; A2ICT2
• Molecular Weight: 94,512 Da
• NCBI Official Full Name: heat shock 70 kDa protein 4L isoform 1
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 4 like
• NCBI Official Symbol: HSPA4L
• NCBI Official Synonym Symbols: APG-1; HSPH3; Osp94
• NCBI Protein Information: heat shock 70 kDa protein 4L
• UniProt Protein Name: Heat shock 70 kDa protein 4L
• Protein Family: Heat shock 70 kDa protein
• UniProt Gene Name: HSPA4L
• UniProt Synonym Gene Names: APG1; OSP94

NCBI Description

The protein encoded by this gene is heat shock inducible and may act as a chaperone. The encoded protein can protect the heat-shocked cell against the harmful effects of aggregated proteins. This gene is highly expressed in leukemia cells and may be a good target for therapeutic intervention. Several transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]

Uniprot Description

Possesses chaperone activity in vitro where it inhibits aggregation of citrate synthase.