Western Blot (WB)
(Western blot analysis of Parasite lysates showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184). Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184) at 1:2000.)
Rabbit Hsp40 Polyclonal Antibody | anti-PF11_0514 antibody
Hsp40 Antibody: ATTO 488
Reactivity
It is species specific to P.falciparum and does nto cross-rect to any protein from human erythrocytes.
Applications
Western Blot, Immunofluorescence
Synonyms
Hsp40; Polyclonal Antibody; Hsp40 Antibody: ATTO 488; PF11_0513; anti-PF11_0514 antibody
Host
Rabbit
Reactivity
It is species specific to P.falciparum and does nto cross-rect to any protein from human erythrocytes.
Clonality
Polyclonal
Form/Format
Protein A Purified
Concentration
1.8mg/mL (varies by lot)
Sequence Length
714
Applicable Applications for anti-PF11_0514 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
1:2000 (WB), 1:50 (IF)
Conjugate
ATTO 488
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide, 0.09% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western blot analysis of Parasite lysates showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184). Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184) at 1:2000.)
Western Blot (WB)
(Western blot analysis of Parasite lysates showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184). Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184) at 1:2000.)
Related Product Information for anti-PF11_0514 antibody
Background Info: This protein A Affinity purified antibody detects an 62kD protein, corresponding to P.falciparum Hsp40 on SDS PAGE immunoblot; in samples from P.Falciparum origin.
Scientific Background: DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s. Hsp40, also known as HDJ1 (1), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-6). Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK (3). PF11_0513 belongs to the Hsp40 family of chaperones. This protein has a Plasmodium export element (PEXEL). It is exported out of the parasite into the infected erythrocytic compartment.
Scientific Background: DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s. Hsp40, also known as HDJ1 (1), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-6). Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK (3). PF11_0513 belongs to the Hsp40 family of chaperones. This protein has a Plasmodium export element (PEXEL). It is exported out of the parasite into the infected erythrocytic compartment.
Product Categories/Family for anti-PF11_0514 antibody
References
1. Ohtsuka, K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240. 2. Melville, M. W. et al. (1997) PNAS USA, 94: 97-102. 3. Hattori, H., Liu, Y-C., Tohnai, I., Ueda, M., Kaneda, T., Kobayashi, T., Tanabe, K., and Ohtsuka, K. (1992) Cell Structure and Function 17: 77-86. 4. Ohtsuka, K. Masuda, A., Nakai, A., and Nagata, K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647. 5. Bardwell, J.C.A., Tilly, K., Craig, E., King, J., Zylicz, M. and Georgopoulos, C. (1986) J. Biol. Chem. 261: 1782-1785. 6. Ohku, M., Tamura, F., Nishimura, S., and Uchida, H. (1986) J. Biol. Chem. 261: 1778-1781.
NCBI and Uniprot Product Information
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Product Notes
The PF11_0514 (Catalog #AAA806616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hsp40 Antibody: ATTO 488 reacts with It is species specific to P.falciparum and does nto cross-rect to any protein from human erythrocytes. and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp40 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). 1:2000 (WB), 1:50 (IF). Researchers should empirically determine the suitability of the PF11_0514 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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