Western Blot (WB)
(Western blot analysis of HSP27 expression in Hela (A), C2C12 (B), mouse brain (C), rat brain (D) whole cell lysates.)
Rabbit HSP27 Polyclonal Antibody | anti-HSPB1 antibody
Anti-HSP27 Antibody
Western Blot (WB)
(Western blot analysis of HSP27 expression in Hela (A), C2C12 (B), mouse brain (C), rat brain (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of HSP27 expression in Hela (A), C2C12 (B), mouse brain (C), rat brain (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of HSP27 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of HSP27 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
Uniprot Description
HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
Protein type: Motility/polarity/chemotaxis; Chaperone; Heat shock protein
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: cytoplasm; cytoskeleton; cytosol; extracellular space; focal adhesion; nucleus; plasma membrane; proteasome complex; spindle; Z disc
Molecular Function: identical protein binding; protein binding; protein kinase binding; protein kinase C binding; protein kinase C inhibitor activity; ubiquitin binding
Biological Process: cell motility; gene expression; negative regulation of apoptosis; negative regulation of protein kinase activity; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of interleukin-1 beta production; positive regulation of tumor necrosis factor biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of mRNA stability; regulation of translational initiation; response to unfolded protein; response to virus; retinal homeostasis; vascular endothelial growth factor receptor signaling pathway
Disease: Charcot-marie-tooth Disease, Axonal, Type 2f; Neuronopathy, Distal Hereditary Motor, Type Iib
Research Articles on HSPB1
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Product Notes
The HSPB1 hspb1 (Catalog #AAA8242451) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HSP27 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSP27 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/1000 - 1/2000); Immunohistochemistry: (1/200 - 1/500). Researchers should empirically determine the suitability of the HSPB1 hspb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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