Rabbit HSL Polyclonal Antibody | anti-HSL antibody

HSL (P548) Polyclonal Antibody

Cat. Num. AAA3000130

• Gene Names: LIPE; HSL; LHS; AOMS4; FPLD6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HSL; Polyclonal Antibody; HSL (P548) Polyclonal Antibody; Hormone sensitive lipase; Hormone sensitive lipase testicular isoform; Hormone-sensitive lipase; LHS; Lipase hormone sensitive; LIPE; LIPS; anti-HSL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HSL (P548) polyclonal antibody detects endogenous levels of HSL protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1076

• Applicable Applications for anti-HSL antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 520-570 of Human HSL.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of HSL (P548) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of HSL (P548) pAb in paraffin-embedded human colorectal cancer carcinoma tissue at 1:100.)

Related Product Information for anti-HSL antibody

HSL (hormone-sensitive lipase), a cytosolic neutral lipase regulated by reversible phosphorylation, catalyzes the rate limiting step in triglyceride lipolysis. HSL hydrolyzes stored triglycerides to free fatty acids in adipose and heart tissues. In organs with steroidogenic tissues, such as small intestine, HSL converts cholesteryl esters to free cholesterol for steroid hormone production. HSL is highly expressed in jejunal enterocytes and in the mucosa of the small intestine. Two major isoforms of HSL have been described resulting from the use of alternative translational start codons. The short isoform is expressed in adipose tissue while the long isoform is expressed in steroidogenic tissues such as testis. The long isoform, often referred to as testicular HSL contains an N-terminus of approximately 300 amino acids not present in the short isoform of HSL.

NCBI and Uniprot Product Information

• NCBI GI #: 21328446
• NCBI GeneID: 3991
• NCBI Accession #: NP_005348.2
• NCBI GenBank Nucleotide #: NP_005348.2
• UniProt Accession #: Q05469
• Molecular Weight: ~ 85, 115kDa
• NCBI Official Full Name: hormone-sensitive lipase
• NCBI Official Synonym Full Names: lipase E, hormone sensitive type
• NCBI Official Symbol: LIPE
• NCBI Official Synonym Symbols: HSL; LHS; AOMS4; FPLD6
• NCBI Protein Information: hormone-sensitive lipase
• UniProt Protein Name: Hormone-sensitive lipase
• UniProt Gene Name: LIPE
• UniProt Synonym Gene Names: HSL
• UniProt Entry Name: LIPS_HUMAN

NCBI Description

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]

Uniprot Description

HSL: hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.

Protein type: EC 3.1.1.79; Lipase

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: lipid particle; caveola; cytosol

Molecular Function: triacylglycerol lipase activity; hormone-sensitive lipase activity; protein binding; protein kinase binding

Biological Process: cholesterol metabolic process; triacylglycerol catabolic process; diacylglycerol catabolic process; protein amino acid phosphorylation; lipid catabolic process; long-chain fatty acid catabolic process

Disease: Abdominal Obesity-metabolic Syndrome 4