Rabbit anti-Human HSD3B1 Polyclonal Antibody | anti-HSD3B1 antibody

Anti-HSD3B1 Antibody

Cat. Num. AAA1753056

• Gene Names: HSD3B1; HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: HSD3B1; Polyclonal Antibody; Anti-HSD3B1 Antibody; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxysteroid 3-dehydrogenase; Delta-5-3-ketosteroid isomerase; Dihydrotestosterone oxidoreductase; Steroid Delta-isomerase; Trophoblast antigen FDO161G; 3BH; HSDB3A; anti-HSD3B1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 373

• Applicable Applications for anti-HSD3B1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.1-0.25ug/ml (Human); IHC-P (Embedded Section): 0.5-1ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
• Immunogen: E Coli-derived human HSD3B1 recombinant protein (Position: Q17-Q373).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-HSD3B1 antibody

Description: Rabbit IgG polyclonal antibody for HSD3B detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human.

Background: HSD3B1 is a human gene that encodes for a 3beta-hydroxysteroid dehydrogenase/delta(5)-delta(4)isomerase type I or hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1. It is mapped to 1p12. The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.

References

1. Taneja SS (January 2017). "Re: HSD3B1 and Resistance to Androgen-Deprivation Therapy in Prostate Cancer: A Retrospective, Multicohort Study". The Journal of Urology. 197 (1): 150.
2. Hearn, Jason (October 2016). "HSD3B1 and resistance to androgen-deprivation therapy in prostate cancer: a retrospective, multicohort study". Lancet Oncology. 17 (10): 1435-1444.
3. Russell AJ, Gaffney D, Gardiner MT, Nickson DA, Sutcliffe RG (March 1991). "Synonymous polymorphism in the coding sequence of human 3-beta hydroxysteroid-5-ene dehydrogenase (HSD)". Nucleic Acids Research. 19 (5): 1172.

NCBI and Uniprot Product Information

• NCBI GI #: 4504507
• NCBI GeneID: 3283
• NCBI Accession #: NP_000853
• NCBI GenBank Nucleotide #: NP_000853.1
• UniProt Accession #: P14060
• NCBI Official Full Name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
• NCBI Official Synonym Full Names: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
• NCBI Official Symbol: HSD3B1
• NCBI Official Synonym Symbols: HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
• NCBI Protein Information: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
• UniProt Protein Name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
• Protein Family: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
• UniProt Gene Name: HSD3B1
• UniProt Synonym Gene Names: 3BH; HSDB3A; 3-beta-HSD I
• UniProt Entry Name: 3BHS1_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids. [provided by RefSeq, Jun 2016]