Western Blot (WB)
((0. 01ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat HSD3B1 Polyclonal Antibody | anti-HSD3B1 antibody
Goat anti-HSD3B1 Antibody
Western Blot: Approx 40kDa band observed in Human Placenta lysates (calculated MW of 42.3kDa according to NP_000853.1). In transfected HEK293 transiently expressing Human HSD3B1 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 01-0.1ug/ml.
Western Blot (WB)
((0. 01ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing Human HSD3B1 (RC204497) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing Human HSD3B1 (RC204497) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
Uniprot Description
HSD3B1: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17- alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone. Belongs to the 3-beta-HSD family.
Protein type: Isomerase; Oxidoreductase; Membrane protein, integral; Lipid Metabolism - androgen and estrogen; Mitochondrial; EC 5.3.3.1; Lipid Metabolism - C21-steroid hormone; EC 1.1.1.145
Chromosomal Location of Human Ortholog: 1p13.1
Cellular Component: endoplasmic reticulum membrane; integral to membrane; mitochondrial inner membrane; mitochondrial intermembrane space; smooth endoplasmic reticulum membrane
Molecular Function: 3-beta-hydroxy-delta5-steroid dehydrogenase activity; steroid delta-isomerase activity
Biological Process: androgen biosynthetic process; estrogen biosynthetic process; glucocorticoid biosynthetic process; mineralocorticoid biosynthetic process; steroid biosynthetic process
Research Articles on HSD3B1
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Product Notes
The HSD3B1 hsd3b1 (Catalog #AAA420114) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HSD3B1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSD3B1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 40kDa band observed in Human Placenta lysates (calculated MW of 42.3kDa according to NP_000853.1). In transfected HEK293 transiently expressing Human HSD3B1 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 01-0.1ug/ml. Researchers should empirically determine the suitability of the HSD3B1 hsd3b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DRHKETLKSK TQ. It is sometimes possible for the material contained within the vial of "HSD3B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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