Rabbit HSD17B10 Polyclonal Antibody | anti-HSD17B10 antibody

HSD17B10 Antibody

Cat. Num. AAA9413836

• Gene Names: HSD17B10; ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; 17b-HSD10; DUPXp11.22
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: HSD17B10; Polyclonal Antibody; HSD17B10 Antibody; ABAD; CAMR; ERAB; HCD2; MHBD; anti-HSD17B10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total HSD17B10 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 252

• Applicable Applications for anti-HSD17B10 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human HSD17B10.
• Target Name: HSD17B10
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HSD17B10 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human thyroid cancer using HSD17B10 antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using HSD17B10 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-HSD17B10 antibody

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

Product Categories/Family for anti-HSD17B10 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 83715985
• NCBI GeneID: 3028
• NCBI Accession #: NP_001032900.1
• NCBI GenBank Nucleotide #: NM_001037811.2
• UniProt Accession #: Q99714; Q5H927; Q6IBS9; Q8TCV9; Q96HD5
• Molecular Weight: 25,984 Da
• NCBI Official Full Name: 3-hydroxyacyl-CoA dehydrogenase type-2 isoform 2
• NCBI Official Synonym Full Names: hydroxysteroid (17-beta) dehydrogenase 10
• NCBI Official Symbol: HSD17B10
• NCBI Official Synonym Symbols: ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; 17b-HSD10; DUPXp11.22
• NCBI Protein Information: 3-hydroxyacyl-CoA dehydrogenase type-2
• UniProt Protein Name: 3-hydroxyacyl-CoA dehydrogenase type-2
• Protein Family: 3-hydroxyacyl-CoA dehydrogenase
• UniProt Gene Name: HSD17B10
• UniProt Synonym Gene Names: ERAB; HADH2; MRPP2; SCHAD; SDR5C1; XH98G2; 17-beta-HSD 10; Mitochondrial RNase P protein 2
• UniProt Entry Name: HCD2_HUMAN

NCBI Description

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Aug 2014]

Uniprot Description

HADH2: Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD). Defects in HSD17B10 are the cause of 2-methyl-3- hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency). MHBD deficiency leads to neurological abnormalities, including psychomotor retardation, and, in virtually all patients, loss of mental and motor skills. Defects in HSD17B10 are the cause of mental retardation syndromic X-linked type 10 (MRXS10). MRXS10 is characterized by mild mental retardation, choreoathetosis and abnormal behavior. A chromosomal microduplication involving HSD17B10 and HUWE1 is the cause of mental retardation X-linked type 17 (MRX17); also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub- average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X- linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non- syndromic X-linked mental retardation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.35; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Oxidoreductase; EC 1.1.1.51; Mitochondrial; EC 1.1.1.178

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: mitochondrion; endoplasmic reticulum; mitochondrial matrix; mitochondrial inner membrane; cytoplasm; plasma membrane

Molecular Function: 3(or 17)beta-hydroxysteroid dehydrogenase activity; protein binding; 7-alpha-hydroxysteroid dehydrogenase activity; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity; 3-hydroxyacyl-CoA dehydrogenase activity

Biological Process: tRNA processing; branched chain family amino acid catabolic process; lipid metabolic process

Disease: Mental Retardation, X-linked, Syndromic 10; 17-beta-hydroxysteroid Dehydrogenase X Deficiency