Immunohistochemistry (IHC)
(Immunohistochemistry with Human Liver cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-HSD11B1 antibody )
Rabbit HSD11B1 Polyclonal Antibody | anti-HSD11B1 antibody
HSD11B1 antibody - N-terminal region
Immunohistochemistry (IHC)
(Immunohistochemistry with Human Liver cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-HSD11B1 antibody )
Immunohistochemistry (IHC)
(Immunohistochemistry with Human Liver cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-HSD11B1 antibody )
Western Blot (WB)
(Host: RatTarget Name: HSD11B1Sample Tissue: Rat LiverAntibody Dilution: 1ug/ml)
Western Blot (WB)
(Host: RatTarget Name: HSD11B1Sample Tissue: Rat LiverAntibody Dilution: 1ug/ml)
Western Blot (WB)
(WB Suggested Anti-HSD11B1 Antibody Titration: 1 ug/mlPositive Control: Fetal liver cell lysate)
Western Blot (WB)
(WB Suggested Anti-HSD11B1 Antibody Titration: 1 ug/mlPositive Control: Fetal liver cell lysate)
Western Blot (WB)
(WB Suggested Anti-HSD11B1 Antibody Titration: 2 ug/mlPositive Control: Transient overexpression lysate of HSD11B1 and Non-overexpressed vector control lysate)
Western Blot (WB)
(WB Suggested Anti-HSD11B1 Antibody Titration: 2 ug/mlPositive Control: Transient overexpression lysate of HSD11B1 and Non-overexpressed vector control lysate)
Target Description: HSD11B1 is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, HSD11B1 can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children.The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2011]
Uniprot Description
HSD11B1: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7- ketocholesterol to 7-beta-hydroxycholesterol. Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD). In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS). Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Endoplasmic reticulum; Oxidoreductase; Lipid Metabolism - C21-steroid hormone; EC 1.1.1.146
Chromosomal Location of Human Ortholog: 1q32-q41
Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane
Molecular Function: 11-beta-hydroxysteroid dehydrogenase activity
Biological Process: steroid metabolic process; glucocorticoid biosynthetic process; lung development
Disease: Cortisone Reductase Deficiency 2
Research Articles on HSD11B1
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Product Notes
The HSD11B1 hsd11b1 (Catalog #AAA3207881) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSD11B1 antibody - N-terminal region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's HSD11B1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the HSD11B1 hsd11b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: QKVVSHCLEL GAASAHYIAG TMEDMTFAEQ FVAQAGKLMG GLDMLILNHI. It is sometimes possible for the material contained within the vial of "HSD11B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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