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Western Blot (WB)
(Anti-Hsc70 antibody, MBS175175, Western blottingLane 1: Rat Testis Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Liver Tissue LysateLane 4: Rat Lung Tissue LysateLane 5: CEM Cell LysateLane 6: HELA Cell LysateLane 7: SMMC Cell LysateLane 8: HT1080 Cell LysateLane 9: COLO320 Cell Lysate )
Rabbit Hsc70 Polyclonal Antibody | anti-HSPA8 antibody
Anti-Hsc70 antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Synonyms
Hsc70; Polyclonal Antibody; Anti-Hsc70 antibody; Heat shock cognate 71 kDa protein; heat shock 70kDa protein 8; 2410008N15Rik antibody; Constitutive heat shock protein 70 antibody; Heat shock 70 kDa protein 8 antibody; Heat shock 70kD protein 10 antibody; Heat shock 70kD protein 8 antibody; Heat shock 70kDa protein 8 antibody; Heat shock cognate 71 kDa protein antibody; Heat shock cognate 71 kDa protein. antibody; Heat shock cognate protein 54 antibody; Heat shock cognate protein 71 kDa antibody; Heat shock cognate protein 71-kDa antibody; Heat shock protein 8 antibody; Heat shock protein A8 antibody; Heat-shock70-KD protein 10; formerly antibody; HSC54 antibody; HSC71 antibody; Hsc73 antibody; HSP71 antibody; HSP73 antibody; HSP7C_HUMAN antibody; HSPA10 antibody; HSPA8 antibody; LAP1 antibody; Lipopolysaccharide associated protein 1 antibody; LPS associated protein 1 antibody; LPS associated protein antibody; MGC102007 antibody; MGC106514 antibody; MGC114311 antibody; MGC118485 antibody; MGC131511 antibody; MGC29929 antibody; N-myristoyltransferase inhibitor protein 71 antibody; NIP71 antibody; anti-HSPA8 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Applicable Applications for anti-HSPA8 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Hsc70 (13-31aa TTYSCVGVFQHGKVEIIAN), identical to the related rat and mouse sequence.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-Hsc70 antibody, MBS175175, Western blottingLane 1: Rat Testis Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Liver Tissue LysateLane 4: Rat Lung Tissue LysateLane 5: CEM Cell LysateLane 6: HELA Cell LysateLane 7: SMMC Cell LysateLane 8: HT1080 Cell LysateLane 9: COLO320 Cell Lysate )
Western Blot (WB)
(Anti-Hsc70 antibody, MBS175175, Western blottingLane 1: Rat Testis Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Liver Tissue LysateLane 4: Rat Lung Tissue LysateLane 5: CEM Cell LysateLane 6: HELA Cell LysateLane 7: SMMC Cell LysateLane 8: HT1080 Cell LysateLane 9: COLO320 Cell Lysate )
Immunohistochemistry (IHC)
(Anti-Hsc70 antibody, MBS175175, IHC(P)IHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-Hsc70 antibody, MBS175175, IHC(P)IHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-Hsc70 antibody, MBS175175, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-Hsc70 antibody, MBS175175, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunocytochemistry (ICC)
(Anti-Hsc70 antibody, MBS175175, ICCICC: HELA Cell )
Immunocytochemistry (ICC)
(Anti-Hsc70 antibody, MBS175175, ICCICC: HELA Cell )
Immunohistochemistry (IHC)
(Anti-Hsc70 antibody, MBS175175, IHC(F)IHC(F): Rat Intestine Tissue )
Immunohistochemistry (IHC)
(Anti-Hsc70 antibody, MBS175175, IHC(F)IHC(F): Rat Intestine Tissue )
Related Product Information for anti-HSPA8 antibody
Description: Rabbit IgG polyclonal antibody for Heat shock cognate 71 kDa protein(HSPA8) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Background: The 70 kilodalton heat shock proteins(Hsp70s) are a family of ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP(Adenosine triphosphate) and hydrolyzes it to ADP(Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. By binding tightly to partially-synthesized peptide sequences(incomplete proteins), Hsp70 prevents them from aggregating and being rendered nonfunctional. And it also can act to protect cells from thermal or oxidative stress. Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins. Interaction with CHIP(Carboxyl-terminus of Hsp70 Interacting Protein)-an E3 ubiquitin ligase-allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.
Background: The 70 kilodalton heat shock proteins(Hsp70s) are a family of ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP(Adenosine triphosphate) and hydrolyzes it to ADP(Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. By binding tightly to partially-synthesized peptide sequences(incomplete proteins), Hsp70 prevents them from aggregating and being rendered nonfunctional. And it also can act to protect cells from thermal or oxidative stress. Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins. Interaction with CHIP(Carboxyl-terminus of Hsp70 Interacting Protein)-an E3 ubiquitin ligase-allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.
NCBI and Uniprot Product Information
UniProt Accession #
Protein Family
Similar Products
Product Notes
The HSPA8 (Catalog #AAA175175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hsc70 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Hsc70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC. Researchers should empirically determine the suitability of the HSPA8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsc70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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