Rabbit Hsc70 Polyclonal Antibody | anti-Hsc70 antibody

Hsc70 Antibody

Cat. Num. AAA9435117

• Gene Names: HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; NIP71; HSPA10
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Purity: Peptide affinity purified
• Synonyms: Hsc70; Polyclonal Antibody; Hsc70 Antibody; 2410008N15Rik antibody; Constitutive heat shock protein 70 antibody; Epididymis luminal protein 33 antibody; Epididymis secretory sperm binding protein Li 72p antibody; Heat shock 70 kDa protein 8 antibody; Heat shock 70kD protein 10 antibody; Heat shock 70kD protein 8 antibody; Heat shock 70kDa protein 8 antibody; Heat shock cognate 71 kDa protein antibody; Heat shock cognate protein 54 antibody; Heat shock cognate protein 71 kDa antibody; Heat shock protein 8 antibody; Heat shock protein A8 antibody; Heat shock protein family A (Hsp70) member 8 antibody; Heat-shock70-KD protein 10; formerly antibody; HEL 33 antibody; HEL S 72p antibody; HSC54 antibody; HSC71 antibody; Hsc73 antibody; HSP71 antibody; HSP73 antibody; HSP7C_HUMAN antibody; HSPA10 antibody; HSPA8 antibody; LAP1 antibody; Lipopolysaccharide associated protein 1 antibody; LPS associated protein 1 antibody; LPS associated protein antibody; MGC102007 antibody; MGC106514 antibody; MGC114311 antibody; MGC118485 antibody; MGC131511 antibody; MGC29929 antibody; N-myristoyltransferase inhibitor protein 71 antibody; NIP71 antibody; anti-Hsc70 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Peptide affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-Hsc70 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; ICC: 1:50-1:200
• Immunogen: Peptide
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of HSC70 on different cell lysate using anti- HSC70 antibody at 1/1, 000 dilution. Positive control�� Lane1: HepG2 Lane2: Hela Lane3: NIH-3T3 Lane4: F9 Lane5: PC-12)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast tissue using anti- HSC70 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- HSC70 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti- HSC70 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- HSC70 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining HSC70 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining HSC70 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining HSC70 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-Hsc70 antibody

The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. All members of the family, except HSP 70, are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. Both HSP 70 and HSC 70 are involved in the chaperoning of nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.

Product Categories/Family for anti-Hsc70 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5729877
• NCBI GeneID: 3312
• NCBI Accession #: NP_006588.1
• NCBI GenBank Nucleotide #: NM_006597.4
• UniProt Accession #: P11142; Q9H3R6
• Molecular Weight: 70,898 Da
• NCBI Official Full Name: heat shock cognate 71 kDa protein isoform 1
• NCBI Official Synonym Full Names: heat shock 70kDa protein 8
• NCBI Official Symbol: HSPA8
• NCBI Official Synonym Symbols: LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; NIP71; HSPA10
• NCBI Protein Information: heat shock cognate 71 kDa protein; LPS-associated protein 1; heat shock 70kd protein 10; heat shock cognate protein 54; constitutive heat shock protein 70; lipopolysaccharide-associated protein 1; N-myristoyltransferase inhibitor protein 71
• UniProt Protein Name: Heat shock cognate 71 kDa protein
• Protein Family: Hsc70-interacting protein
• UniProt Gene Name: HSPA8
• UniProt Synonym Gene Names: HSC70; HSP73; HSPA10
• UniProt Entry Name: HSP7C_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Chaperone; Heat shock protein; Nucleolus

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: nucleoplasm; spliceosome; extracellular space; focal adhesion; membrane; melanosome; nucleolus; plasma membrane; intracellular; ribonucleoprotein complex; nucleus; cytosol; ubiquitin ligase complex

Molecular Function: protein binding; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; ubiquitin protein ligase binding; ATPase activity; unfolded protein binding; ATPase activity, coupled; ATP binding

Biological Process: axon guidance; viral reproduction; chaperone cofactor-dependent protein folding; protein folding; transcription, DNA-dependent; regulation of cell cycle; neurotransmitter secretion; RNA splicing; response to unfolded protein; nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; synaptic transmission; gene expression; protein refolding; post-Golgi vesicle-mediated transport; negative regulation of transcription, DNA-dependent