Rabbit HS1 Polyclonal Antibody | anti-HS1 antibody

HS1 Antibody

Cat. Num. AAA9611014

• Gene Names: HCLS1; HS1; p75; CTTNL; lckBP1
• Reactivity: Human; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: HS1; Polyclonal Antibody; HS1 Antibody; Cortactin like; CTTNL; HCLS 1; Hcls1; HCLS1_HUMAN; Hematopoietic cell specific Lyn substrate 1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; HS 1; LckBP1; OTTHUMP00000215180; OTTHUMP00000215182; p75; anti-HS1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HS1 Antibody detects endogenous levels of HS1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 486

• Applicable Applications for anti-HS1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human HS1.
• Tissue Specificity: Expressed only in tissues and cells of hematopoietic origin.
• Subcellular Location: Mitochondrion
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of HS1 in lysates of JK,HepG2, using HS1 Antibody.)

Related Product Information for anti-HS1 antibody

Description: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.

Post Translational Modifications: Phosphorylated by FES (By similarity). Phosphorylated by LYN, FYN and FGR after cross-linking of surface IgM on B-cells. Phosphorylation by LYN, FYN and FGR requires prior phosphorylation by SYK or FES.

Subunit Structure: Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS (By similarity). Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54 (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 167234422
• NCBI GeneID: 3059
• NCBI Accession #: NP_005326.2
• NCBI GenBank Nucleotide #: NP_005326.2
• UniProt Accession #: P14317
• Molecular Weight: Observed: 78 kDa; Predicted: 55 kDa
• NCBI Official Full Name: hematopoietic lineage cell-specific protein isoform 1
• NCBI Official Synonym Full Names: hematopoietic cell-specific Lyn substrate 1
• NCBI Official Symbol: HCLS1
• NCBI Official Synonym Symbols: HS1; p75; CTTNL; lckBP1
• NCBI Protein Information: hematopoietic lineage cell-specific protein
• UniProt Protein Name: Hematopoietic lineage cell-specific protein
• Protein Family: Stress-response A/B barrel domain-containing protein
• UniProt Gene Name: HCLS1
• UniProt Synonym Gene Names: HS1
• UniProt Entry Name: HCLS1_HUMAN

Uniprot Description

HS1: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS. Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54. Expressed only in tissues and cells of hematopoietic origin.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3q13

Cellular Component: transcription factor complex; membrane; mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; protein complex binding; actin binding; protein kinase binding; SH3 domain binding

Biological Process: positive regulation of granulocyte differentiation; response to hormone stimulus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; regulation of actin filament polymerization; regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of STAT protein; actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of macrophage differentiation