Immunohistochemistry (IHC)
(MBS9603510 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit HRP2 Polyclonal Antibody | anti-HRP2 antibody
HRP2 Antibody
Gene Names
HDGFL2; HRP2; HDGF2; HRP-2; HDGF-2; HDGFRP2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
HRP2; Polyclonal Antibody; HRP2 Antibody; HDGF-2; HDGF2; Hdgfrp2; HDGR2_HUMAN; Hepatoma-derived growth factor 2; Hepatoma-derived growth factor; Hepatoma-derived growth factor-related protein 2; heptoma derived growth factor related protein 2; HRP-2; anti-HRP2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HRP2 antibody detects endogenous levels of HRP2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
671
Applicable Applications for anti-HRP2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human HRP2.
Subcellular Location
Nucleus
Tissue Specificity
Widely expressed. High expression is found in heart, skeletal muscle, ovary and testis. Overexpression is frequently observed in hepatocellular carcinoma samples.
Predicted Cross Reactivity
Pig, Sheep, Dog, Xenopus
Similarity
Pig (100%), Sheep (100%), Dog (100%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603510 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603510 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2, using HRP2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2, using HRP2 antibody.)
Related Product Information for anti-HRP2 antibody
Function: Involved in cellular growth control, through the regulation of cyclin D1 expression.
Subunit Structure: Interacts with HDGF (By similarity). Interacts with trimethylated 'Lys-36' of histone H3 (H3K36me3). Interacts with trimethylated 'Lys-79' of histone H3 (H3K79me3), but has higher affinity for H3K36me3. Interacts with IWS1 (PubMed:25689719).
Similarity: Belongs to the HDGF family.
Subunit Structure: Interacts with HDGF (By similarity). Interacts with trimethylated 'Lys-36' of histone H3 (H3K36me3). Interacts with trimethylated 'Lys-79' of histone H3 (H3K79me3), but has higher affinity for H3K36me3. Interacts with IWS1 (PubMed:25689719).
Similarity: Belongs to the HDGF family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 74 kDa
Predicted: 75 kDa
Predicted: 75 kDa
NCBI Official Full Name
hepatoma-derived growth factor-related protein 2 isoform 1
NCBI Official Synonym Full Names
HDGF like 2
NCBI Official Symbol
HDGFL2
NCBI Official Synonym Symbols
HRP2; HDGF2; HRP-2; HDGF-2; HDGFRP2
NCBI Protein Information
hepatoma-derived growth factor-related protein 2
UniProt Protein Name
Hepatoma-derived growth factor-related protein 2
UniProt Gene Name
HDGFL2
UniProt Synonym Gene Names
HDGF-related protein 2; HRP-2; HDGF-2
NCBI Description
This gene encodes a member of the hepatoma-derived growth factor (HDGF) family. The protein includes an N-terminal PWWP domain that binds to methyl-lysine-containing histones, with specific binding of this protein to tri-methylated lysines 36 and 79 of histone H3, and di- and tri-methylated lysine 20 of histone H4. The protein functions in LEDGF/p75-independent HIV-1 replication by determining HIV-1 integration site selection. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]
Uniprot Description
Involved in cellular growth control, through the regulation of cyclin D1 expression.
Research Articles on HRP2
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Product Notes
The HRP2 hdgfl2 (Catalog #AAA9603510) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HRP2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HRP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the HRP2 hdgfl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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