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Western Blot (WB) (Figure 1: Western blot analysis using GATA1 mouse mAb against K562 (1) cell lysate.)

Mouse anti-Human GATA1 Antibody | anti-GATA1 antibody

GATA1 Antibody

Gene Names
GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Synonyms
GATA1; GATA1 Antibody; erythroid specific transcription factor; GATA sequence binding protein (same as EryF1; NF-E1; EF-1; EF gamma-a; GF1); expressed in RBC; mast cell; megakaryocyte; hematopoietic progenitor cell; testis; associated with a special class of nuclear bodies; activated by erythropoietin; Inactivated by coactivation of DEATH receptors (TNFRSF6) mediated caspase cleavage Tissue specificity: Erythrocytes; anti-GATA1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
4F5
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
413
Applicable Applications for anti-GATA1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of human VCAM1 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.

Western Blot (WB)

(Figure 1: Western blot analysis using GATA1 mouse mAb against K562 (1) cell lysate.)

Western Blot (WB) (Figure 1: Western blot analysis using GATA1 mouse mAb against K562 (1) cell lysate.)

Immunofluorescence (IF)

(Figure 2: Immunofluorescence analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.)

Immunofluorescence (IF) (Figure 2: Immunofluorescence analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.)

Immunohistochemistry (IHC)

(Figure 3: Immunohistochemical analysis of paraffin-embedded pancreatic cancer, using GATA1 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Figure 3: Immunohistochemical analysis of paraffin-embedded pancreatic cancer, using GATA1 mouse mAb with DAB staining.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42.7
NCBI Official Full Name
erythroid transcription factor
NCBI Official Synonym Full Names
GATA binding protein 1
NCBI Official Symbol
GATA1
NCBI Official Synonym Symbols
GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
NCBI Protein Information
erythroid transcription factor
UniProt Protein Name
Erythroid transcription factor
Protein Family
UniProt Gene Name
GATA1
UniProt Synonym Gene Names
ERYF1; GF1; GATA-1; GF-1

NCBI Description

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781).

Research Articles on GATA1

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Product Notes

The GATA1 gata1 (Catalog #AAA850607) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GATA1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GATA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 IF: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the GATA1 gata1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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