Rabbit Hrk Polyclonal Antibody | anti-Hrk antibody

Hrk (Activator of Apoptosis Harakiri, BH3-interacting Domain Containing Protein 3, BID3, DP5, Harakiri BCL2 Interacting Protein (Contains Only BH3 Domain), Neuronal Death Protein DP5) (MaxLight 750)

Cat. Num. AAA6481160

• Gene Names: HRK; DP5; HARAKIRI
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: Hrk; Polyclonal Antibody; Hrk (Activator of Apoptosis Harakiri; BH3-interacting Domain Containing Protein 3; BID3; DP5; Harakiri BCL2 Interacting Protein (Contains Only BH3 Domain); Neuronal Death Protein DP5) (MaxLight 750); Activator of Apoptosis Hrk; Human Brain 3UTR of mRNA for Neuronal Death Protein Partial Sequence; anti-Hrk antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Harakiri (HRK), also known as DP5. HRK is a BH3 only protein of the Bcl-2 family, which promotes Bax dependent apoptosis. Species Crossreactivity: mouse and rat.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight750.

• Applicable Applications for anti-Hrk antibody: Western Blot (WB)
• Application Notes: WB: 2-5ug/ml. Detects a band at ~13kD in mouse pancreas tissue lysate.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to 15aa near the center of human HRK (NP_003797).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-Hrk antibody

Antibodies; Apoptosis

References

1. Nakamura M. et al. (2005). Frequent HRK inactivation associated with low apoptotic index in secondary glioblastomas. Acta Neuropathol (Berl). 110: 402-410. 2. Sanz, C. et al. (2001). Interleukin 3-dependent activation of DREAM is involved in transcriptional silencing of the apoptotic Hrk gene in hematopoietic progenitor cells. EMBO J. 20: 2286-2292. 3. Sunayama, J. et al. (2004). Physical and functional interaction between BH3-only protein Hrk and mitochondrial pore-forming protein p32. Cell Death Differ. 11: 771-781.

NCBI and Uniprot Product Information

• NCBI GI #: 4504493
• NCBI GeneID: 8739
• NCBI Accession #: NP_003797
• NCBI GenBank Nucleotide #: NM_003806.2
• UniProt Accession #: O00198
• Molecular Weight: 9,884 Da
• NCBI Official Full Name: activator of apoptosis harakiri
• NCBI Official Synonym Full Names: harakiri, BCL2 interacting protein
• NCBI Official Symbol: HRK
• NCBI Official Synonym Symbols: DP5; HARAKIRI
• NCBI Protein Information: activator of apoptosis harakiri; death protein 5; BCL2-interacting protein; activator of apoptosis Hrk; neuronal death protein DP5; BH3-interacting domain-containing protein 3; harakiri, BCL2 interacting protein (contains only BH3 domain)
• UniProt Protein Name: Activator of apoptosis harakiri
• Protein Family: Activator of apoptosis harakiri
• UniProt Gene Name: HRK
• UniProt Synonym Gene Names: BID3
• UniProt Entry Name: HRK_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. Members of this family are involved in activating or inhibiting apoptosis. The encoded protein localizes to intracellular membranes. This protein promotes apoptosis by interacting with the apoptotic inhibitors BCL-2 and BCL-X(L) via its BH3 domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

Uniprot Description

HRK: Promotes apoptosis.

Protein type: Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: mitochondrion; integral to membrane

Molecular Function: protein binding

Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of protein homooligomerization