Western Blot (WB)
(Western Blot analysis of A549 cells with EIF2AK1 Polyclonal Antibody.)
Rabbit HRI Polyclonal Antibody | anti-HRI antibody
EIF2AK1 Polyclonal Antibody
Reactivity
Human, Mouse, Monkey
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity purification
Synonyms
HRI; Polyclonal Antibody; EIF2AK1 Polyclonal Antibody; EIF2AK1; KIAA1369; Eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2; anti-HRI antibody
Host
Rabbit
Reactivity
Human, Mouse, Monkey
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Applicable Applications for anti-HRI antibody
Western Blot (WB), Immunohistochemistry-paraffin (IHC-p), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:100-1:300
ELISA: 1:40000
IHC: 1:100-1:300
ELISA: 1:40000
Immunogen
Synthesized peptide derived from the C-terminal region of human HRI
Note
Centrifuge before opening to ensure complete recovery of vial contents.
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of A549 cells with EIF2AK1 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of A549 cells with EIF2AK1 Polyclonal Antibody.)
Related Product Information for anti-HRI antibody
Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
Calculated MW: 71kDa
Observed MW: 71kDa
Observed MW: 71kDa
Similar Products
Product Notes
The HRI (Catalog #AAA2538144) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF2AK1 Polyclonal Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's HRI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-paraffin (IHC-p), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:100-1:300 ELISA: 1:40000. Researchers should empirically determine the suitability of the HRI for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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