Rabbit EIF2AK1 Polyclonal Antibody | anti-EIF2AK1 antibody

anti-EIF2AK1 antibody

Cat. Num. AAA7611921

• Gene Names: EIF2AK1; HCR; HRI
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE. Immunogen affinity purified
• Synonyms: EIF2AK1; Polyclonal Antibody; anti-EIF2AK1 antibody; HCR; HRI; eukaryotic translation initiation factor 2-alpha kinase 1

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE. Immunogen affinity purified
• Form/Format: Liquid. PBS with 0.02% sodium azide and 50% glycerol pH7.3

• Applicable Applications for anti-EIF2AK1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: eukaryotic translation initiation factor 2-alpha kinase 1
• Preparation and Storage: Store at -20 degree C for 12 months. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human colon using MBS7611921(EIF2AK1 antibody) at dilution of 1:100)

Western Blot (WB)

(HepG2 cells were subjected to SDS PAGE followed by western blot with MBS7611921(EIF2AK1 antibody) at dilution of 1:1000)

Related Product Information for anti-EIF2AK1 antibody

Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells(RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions(By similarity).

Product Categories/Family for anti-EIF2AK1 antibody

Neuroscience; Immunology; Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 197245437
• NCBI GeneID: 27102
• NCBI Accession #: NP_001127807.1
• NCBI GenBank Nucleotide #: NM_001134335.1
• UniProt Accession #: Q9BQI3; Q549K6; Q8NBW3; Q9HC02; Q9NYE0; Q9P0V6; Q9P1J5; Q9P2H8; Q9UHG4; A8K2R2
• Molecular Weight: 71,106 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 2-alpha kinase 1 isoform b
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 1
• NCBI Official Symbol: EIF2AK1
• NCBI Official Synonym Symbols: HCR; HRI
• NCBI Protein Information: eukaryotic translation initiation factor 2-alpha kinase 1; heme-regulated inhibitor; heme-regulated repressor; heme-controlled repressor; heme sensitive initiation factor 2a kinase; heme regulated initiation factor 2 alpha kinase; heme-regulated initiatio
• UniProt Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 1
• Protein Family: Eukaryotic translation initiation factor 2-alpha kinase
• UniProt Gene Name: EIF2AK1
• UniProt Synonym Gene Names: HRI; KIAA1369; HCR
• UniProt Entry Name: E2AK1_HUMAN

NCBI Description

The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

HRI: a protein kinase of the PEK family. Phosphorylates the alpha subunit of eukaryotic initiation factor 2 in response to various stress conditions, inhibiting protein synthesis at the level of initiation. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. Binding of nitric oxide (NO) to the heme iron in the N-terminal heme-binding domain activates the kinase activity, while binding of carbon monoxide (CO) suppresses kinase activity. Requires association with a multiprotein complex containing Hsp90, CDC37 and PPP5C for maturation and activation by autophosporylation. Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.11.1; Translation; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; PEK family

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cytoplasm

Molecular Function: protein homodimerization activity; heme binding; eukaryotic translation initiation factor 2alpha kinase activity; ATP binding

Biological Process: response to external stimulus; negative regulation of cell proliferation; negative regulation of translational initiation by iron; protoporphyrinogen IX metabolic process; protein amino acid autophosphorylation; response to stress; negative regulation of hemoglobin biosynthetic process