Western Blot (WB)
(Western blot analysis of HRB expression in AGFG1 transfected 293T cells, The lane on the left is treated with the antigen-specific peptide.)
Rabbit HRB Polyclonal Antibody | anti-HRB antibody
HRB Antibody
Gene Names
AGFG1; HRB; RAB; RIP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
HRB; Polyclonal Antibody; HRB Antibody; AGFG1; AGFG1_HUMAN; Arf-GAP domain and FG repeats-containing protein 1; ArfGAP with FG repeats 1; AU045498; C130049H11Rik; C85612; D730048C23Rik; DKFZp686I15205; HIV 1 Rev binding protein; HIV-1 Rev-binding protein; MGC116375; MGC116938; MGC116940; Nucleoporin like protein RIP; Nucleoporin-like protein RIP; RAB; Rev interacting protein; Rev-interacting protein; Rev/Rex activation domain-binding protein; RIP; anti-HRB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HRB antibody detects endogenous levels of total HRB
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
584
Applicable Applications for anti-HRB antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Subcellular Location
Nucleus. Cytoplasmic Vesicle.
Tissue Specificity
Ubiquitously expressed.
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of HRB expression in AGFG1 transfected 293T cells, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of HRB expression in AGFG1 transfected 293T cells, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-HRB antibody
Description: Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.
Subunit Structure: Interacts with EPS15R and EPS15. Interacts with FCHO1.
Post-translational Modifications: O-glycosylated.
Similarity: Contains FG repeats. The FG repeat region is required for acting as a cofactor of HIV-1 Rev.
Subunit Structure: Interacts with EPS15R and EPS15. Interacts with FCHO1.
Post-translational Modifications: O-glycosylated.
Similarity: Contains FG repeats. The FG repeat region is required for acting as a cofactor of HIV-1 Rev.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 58 kDa
Predicted: 59 kDa
Predicted: 59 kDa
NCBI Official Full Name
arf-GAP domain and FG repeat-containing protein 1 isoform 1
NCBI Official Synonym Full Names
ArfGAP with FG repeats 1
NCBI Official Symbol
AGFG1
NCBI Official Synonym Symbols
HRB; RAB; RIP
NCBI Protein Information
arf-GAP domain and FG repeat-containing protein 1
UniProt Protein Name
Arf-GAP domain and FG repeat-containing protein 1
Protein Family
UniProt Gene Name
AGFG1
UniProt Synonym Gene Names
HRB; RAB; RIP
NCBI Description
The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
Uniprot Description
Required for vesicle docking or fusion during acrosome biogenesis (). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.
Research Articles on HRB
Similar Products
Product Notes
The HRB agfg1 (Catalog #AAA9604153) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HRB Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HRB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the HRB agfg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
