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IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using PSAP antibody. Blue: DAPI for nuclear staining.)
Rabbit PSAP Polyclonal Antibody | anti-PSAP antibody
PSAP Polyclonal Antibody
Gene Names
PSAP; GLBA; SAP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
PSAP; Polyclonal Antibody; PSAP Polyclonal Antibody; FLJ00245; GLBA; MGC110993; SAP1; anti-PSAP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
524
Applicable Applications for anti-PSAP antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human PSAP
Calculated Molecular Weight
58kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using PSAP antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using PSAP antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using PSAP Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using PSAP Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using PSAP Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using PSAP Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver using PSAP Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver using PSAP Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat brain using PSAP Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat brain using PSAP Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using PSAP Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using PSAP Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver using PSAP Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver using PSAP Antibody at dilution of 1:200 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PSAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PSAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-PSAP antibody
The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. They are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. (PMID: 2001789) Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy (PubMed: 2060627, PMID: 15773042). This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.
Product Categories/Family for anti-PSAP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
524
NCBI Official Full Name
Prosaposin
NCBI Official Synonym Full Names
prosaposin
NCBI Official Symbol
PSAP
NCBI Official Synonym Symbols
GLBA; SAP1
NCBI Protein Information
prosaposin; proactivator polypeptide; sphingolipid activator protein-1
UniProt Protein Name
Prosaposin
UniProt Gene Name
PSAP
UniProt Synonym Gene Names
GLBA; SAP1; CSAct; SAP-1; SAP-2
UniProt Entry Name
SAP_HUMAN
Similar Products
Product Notes
The PSAP psap (Catalog #AAA10696) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PSAP Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PSAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the PSAP psap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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