Rabbit anti-Human HRAS Polyclonal Antibody | anti-HRAS antibody

HRAS (HRAS1, H-Ras-1, c-H-ras, C-Ha-Ras1, Ha-Ras, GTPase HRas, C-Bas/Has, CTLO, HAMSV, H-RASIDX, K-Ras, p21ras, RASH1, Transforming Protein p21) (APC)

Cat. Num. AAA6481273

• Gene Names: HRAS; CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: HRAS; Polyclonal Antibody; HRAS (HRAS1; H-Ras-1; c-H-ras; C-Ha-Ras1; Ha-Ras; GTPase HRas; C-Bas/Has; CTLO; HAMSV; H-RASIDX; K-Ras; p21ras; RASH1; Transforming Protein p21) (APC); v-Ha-ras Harvey Rat Sarcoma Viral Oncogene Homolog; N-RAS; anti-HRAS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HRAS.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-HRAS antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human HRAS (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-HRAS Antibody (Center) in Jurkat cell line lysates (35ug/lane). HRAS (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with HRAS antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HRAS Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-HRAS antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Winter-Vann, A.M., Proc. Natl. Acad. Sci. USA 100(11), 6529-6534 (2003). 2. Coats, S.G., Biochemistry 38(39), 12926-12934 (1999). 3. Sakai, E., Int. J. Cancer 52(6), 867-872 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4885425
• NCBI GeneID: 3265
• NCBI Accession #: NP_005334
• NCBI GenBank Nucleotide #: NM_005343.2
• UniProt Accession #: P01112; Q14080; Q6FHV9; Q9BR65; Q9UCE2; B5BUA0
• Molecular Weight: 18,870 Da
• NCBI Official Full Name: GTPase HRas isoform 1
• NCBI Official Synonym Full Names: Harvey rat sarcoma viral oncogene homolog
• NCBI Official Symbol: HRAS
• NCBI Official Synonym Symbols: CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
• NCBI Protein Information: GTPase HRas
• UniProt Protein Name: GTPase HRas
• Protein Family: HRAS-like suppressor
• UniProt Gene Name: HRAS
• UniProt Synonym Gene Names: HRAS1
• UniProt Entry Name: RASH_HUMAN

NCBI Description

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HRas: a small GTPase protein of the Ras family. Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Mutations are implicated in a variety of human tumors.

Protein type: Motility/polarity/chemotaxis; G protein, monomeric, Ras; Oncoprotein; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: Golgi membrane; Golgi apparatus; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein binding; GTP binding

Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein heterooligomerization; positive regulation of JNK cascade; signal transduction; chemotaxis; negative regulation of cell proliferation; positive regulation of MAP kinase activity; synaptic transmission; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; small GTPase mediated signal transduction; positive regulation of cell proliferation; ephrin receptor signaling pathway; visual learning; negative regulation of neuron apoptosis; cell cycle arrest; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; mitotic cell cycle checkpoint; MAPKKK cascade; endocytosis; social behavior; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; cell proliferation; organ morphogenesis; negative regulation of cell differentiation; Ras protein signal transduction; insulin receptor signaling pathway; striated muscle cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; blood coagulation; leukocyte migration; positive regulation of epithelial cell proliferation; positive regulation of DNA replication; positive regulation of cell migration

Disease: Bladder Cancer; Thyroid Carcinoma, Follicular; Costello Syndrome; Schimmelpenning-feuerstein-mims Syndrome; Melanocytic Nevus Syndrome, Congenital; Nevus, Epidermal