Rabbit HOXB8 Polyclonal Antibody | anti-Hoxb8 antibody

Anti-HOXB8 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102034

• Gene Names: HOXB8; HOX2; HOX2D; Hox-2.4
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: HOXB8; Polyclonal Antibody; Anti-HOXB8 Antibody; Rabbit Polyclonal; Hoxb8 Antibody; Rabbit PAb; Antigen Affinity Purified; homeobox B8; Anti-Hox-2.4 Antibody; Anti-HOX2 Antibody; Anti-HOX2D Antibody; anti-Hoxb8 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human HOXB8
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-Hoxb8 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human Hoxb8
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Hoxb8, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-HOXB8 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: HL60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:32 kDa)

Related Product Information for anti-Hoxb8 antibody

Homeobox B8 (HOXB8) is a member of the HOX family. HOXB8 functions as a sequence-specific transcription factor that is involved in development. Increased expression of this gene is associated with a wide variety of tumors. HOXB8 plays an important role in the development and metastasis of gastric carcinoma. HoxB8 was the first homeobox gene identified as a cause of leukemia. Hoxb8 overexpression immortalizes hematopoietic progenitor cells in a growth-factor-dependant manner and co-operates with interleukin-3 (IL-3) to cause acute myeloid leukemia. Ectopic expression of a single HoxB gene, HoxB8, in mesencephalic neural crest results in a strongly increased expression of sympathetic neuron characteristics like the transcription factor Hand2, tyrosine hydroxylase (TH), and dopamine-beta-hydroxylase (DBH) in ciliary neurons. HoxB8 has only minor effects in postmitotic ciliary neurons and is unable to induce TH and DBH in the enteric nervous system.

References

Salmanidis M, et al. (2013) Hoxb8 regulates expression of micrornas to control cell death and differentiation. Cell Death Differ 20 (10): 1370-1380.Knoepfler PS, et al. (2001) Hoxb8 requires its pbx-interaction motif to block differentiation of primary myeloid progenitors and of most cell line models of myeloid differentiation. Oncogene 20 (39): 5440-5448.Huber L, et al. (2012) Hoxb8 in noradrenergic specification and differentiation of the autonomic nervous system. Dev Biol 363 (1): 219-233.Ding WJ, et al. (2017) Hoxb8 promotes tumor metastasis and the epithelial-mesenchymal transition via zeb2 targets in gastric cancer. J Cancer Res Clin Oncol 143 (3): 385-397.

NCBI and Uniprot Product Information

• NCBI GI #: 13273317
• NCBI GeneID: 3218
• NCBI Accession #: NP_076921.1
• NCBI GenBank Nucleotide #: NM_024016.3
• UniProt Accession #: P17481; Q9H1I2
• Molecular Weight: 27,574 Da
• NCBI Official Full Name: homeobox protein Hox-B8
• NCBI Official Synonym Full Names: homeobox B8
• NCBI Official Symbol: HOXB8
• NCBI Official Synonym Symbols: HOX2; HOX2D; Hox-2.4
• NCBI Protein Information: homeobox protein Hox-B8; homeo box 2D; homeo box B8; homeobox protein Hox-2D; homeobox protein Hox-2.4
• UniProt Protein Name: Homeobox protein Hox-B8
• Protein Family: Homeobox protein
• UniProt Gene Name: HOXB8
• UniProt Synonym Gene Names: HOX2D
• UniProt Entry Name: HXB8_HUMAN

NCBI Description

This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development. Increased expression of this gene is associated with colorectal cancer. Mice that have had the murine ortholog of this gene knocked out exhibit an excessive pathologic grooming behavior. This behavior is similar to the behavior of humans suffering from the obsessive-compulsive spectrum disorder trichotillomania. [provided by RefSeq, Jul 2008]

Uniprot Description

HOXB8: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Belongs to the Antp homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: grooming behavior; anterior/posterior pattern formation; dorsal spinal cord development; negative regulation of myeloid cell differentiation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; adult locomotory behavior; sensory perception of pain; embryonic skeletal morphogenesis