Western Blot (WB)
((1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat HOXB6 Polyclonal Antibody | anti-HOXB6 antibody
Goat anti-HOXB6 Antibody
Immunohistochemistry: In paraffin embedded Human Lung shows nuclear staining in the alvioli. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 26kDa band observed in lysates of cell lines HEK293, CACO-2 and MRC5 PD19 (calculated MW of 25.4kDa according to NP_061825.2). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the Antp homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development, including that of lung and skin, and has been localized to both the nucleus and cytoplasm. Altered expression of this gene or a change in the subcellular localization of its protein is associated with some cases of acute myeloid leukemia and colorectal cancer. [provided by RefSeq, Jul 2008]
Uniprot Description
HOXB6: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Belongs to the Antp homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 17q21.3
Cellular Component: nucleus
Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity
Biological Process: anterior/posterior pattern formation; embryonic skeletal morphogenesis; erythrocyte homeostasis; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on HOXB6
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Product Notes
The HOXB6 hoxb6 (Catalog #AAA423251) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HOXB6 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's HOXB6 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 1000. Immunohistochemistry: In paraffin embedded Human Lung shows nuclear staining in the alvioli. Recommended concentration, 5-10ug/ml. Western Blot: Approx 26kDa band observed in lysates of cell lines HEK293, CACO-2 and MRC5 PD19 (calculated MW of 25.4kDa according to NP_061825.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the HOXB6 hoxb6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EPRKSDCAQD KS. It is sometimes possible for the material contained within the vial of "HOXB6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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