Rabbit anti-Human HOXA10 Polyclonal Antibody | anti-HOXA10 antibody

HOXA10, ID (HOXA10, HOX1H, Homeobox protein Hox-A10, Homeobox protein Hox-1.8, Homeobox protein Hox-1H, PL) (APC)

Cat. Num. AAA6307661

• Gene Names: HOXA10; PL; HOX1; HOX1H; HOX1.8
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HOXA10; Polyclonal Antibody; ID (HOXA10; HOX1H; Homeobox protein Hox-A10; Homeobox protein Hox-1.8; Homeobox protein Hox-1H; PL) (APC); PL; anti-HOXA10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-HOXA10 antibody: Immunofluorescence (IF), FLISA, Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HOXA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-262 amino acids from the Central region of human HOXA10.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HOXA10 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Western Blot (WB)

(HOXA10 Antibody (Center) western blot analysis in WiDr,HepG2 cell line lysates (35ug/lane).This demonstrates the HOXA10 antibody detected the HOXA10 protein (arrow).)

Related Product Information for anti-HOXA10 antibody

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor that may regulate gene expression, morphogenesis, and differentiation. More specifically, it may function in fertility, embryo viability, and regulation of hematopoietic lineage commitment. Alternatively spliced transcript variants encoding different isoforms have been described.

Product Categories/Family for anti-HOXA10 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Du, H., et al. Biol. Reprod. 83(2):205-211(2010). Cheng, W., et al. J. Cancer Res. Clin. Oncol. 136(8):1221-1227(2010). Liatsikos, S.A., et al. Reprod. Biomed. Online 21(1):126-132(2010). Godbole, G., et al. J. Reprod. Immunol. 85(2):130-139(2010). Ko, S.Y., et al. Mol. Cell. Endocrinol. 317 (1-2), 112-119 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 182765442
• NCBI GeneID: 3206
• NCBI Accession #: NP_061824.3
• NCBI GenBank Nucleotide #: NM_018951.3
• UniProt Accession #: P31260; O43370; O43605; Q15949; Q504T1
• Molecular Weight: 11,452 Da
• NCBI Official Full Name: homeobox protein Hox-A10
• NCBI Official Synonym Full Names: homeobox A10
• NCBI Official Symbol: HOXA10
• NCBI Official Synonym Symbols: PL; HOX1; HOX1H; HOX1.8
• NCBI Protein Information: homeobox protein Hox-A10; homeo box A10; homeobox protein 1H; homeobox protein HOXA10; homeobox protein Hox-1H; homeobox protein Hox-1.8
• UniProt Protein Name: Homeobox protein Hox-A10
• Protein Family: Homeobox protein
• UniProt Gene Name: HOXA10
• UniProt Synonym Gene Names: HOX1H
• UniProt Entry Name: HXA10_HUMAN

NCBI Description

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor that may regulate gene expression, morphogenesis, and differentiation. More specifically, it may function in fertility, embryo viability, and regulation of hematopoietic lineage commitment. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the downstream homeobox A9 (HOXA9) gene. [provided by RefSeq, Mar 2011]

Uniprot Description

HOXA10: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to the DNA sequence 5'-AA[AT]TTTTATTAC-3'. Belongs to the Abd-B homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7p15.2

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; histone deacetylase binding

Biological Process: anterior/posterior pattern formation; transcription, DNA-dependent; multicellular organismal development; single fertilization; male gonad development; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; uterus development; skeletal development; proximal/distal pattern formation; embryonic limb morphogenesis