Rabbit anti-Human, Mouse Homer 3 Polyclonal Antibody | anti-HOMER3 antibody

Homer 3 (Homer Protein Homolog 3, HOMER3, Homer-3, VESL3) (APC)

Cat. Num. AAA6481097

• Gene Names: HOMER3; VESL3; HOMER-3
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Homer 3; Polyclonal Antibody; Homer 3 (Homer Protein Homolog 3; HOMER3; Homer-3; VESL3) (APC); Homer Homolog 3 (Drosophila); anti-HOMER3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HOMER3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 361

• Applicable Applications for anti-HOMER3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human HOMER3 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HOMER3 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane).HOMER3 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of HOMER3 Antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane).HOMER3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue with HOMER3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of SK-Br-3 cells using HOMER3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of SK-Br-3 cells using HOMER3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-HOMER3 antibody

Antibodies; Neuroscience

References

1. Norton, N., et.al., Am. J. Med. Genet. B Neuropsychiatr. Genet. 120B(1), 18-21 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 224809408
• NCBI GeneID: 9454
• NCBI Accession #: NP_004829.3
• NCBI GenBank Nucleotide #: NP_004829.3
• UniProt Accession #: Q9NSC5
• Molecular Weight: 39.836
• NCBI Official Full Name: homer protein homolog 3 isoform 1
• NCBI Official Synonym Full Names: homer scaffold protein 3
• NCBI Official Symbol: HOMER3
• NCBI Official Synonym Symbols: VESL3; HOMER-3
• NCBI Protein Information: homer protein homolog 3
• UniProt Protein Name: Homer protein homolog 3
• Protein Family: Homer protein
• UniProt Gene Name: HOMER3
• UniProt Synonym Gene Names: Homer-3
• UniProt Entry Name: HOME3_HUMAN

NCBI Description

This gene encodes a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

HOMER3: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses. Belongs to the Homer family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: postsynaptic membrane; cytoplasm; plasma membrane; cell junction; cytosol

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; metabotropic glutatmate receptor binding

Biological Process: metabotropic glutamate receptor signaling pathway; protein targeting