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Western Blot (WB) ((0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat Homeobox B13 Polyclonal Antibody | anti-HOXB13 antibody

Goat anti-Homeobox B13 Antibody

Gene Names
HOXB13; PSGD
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Homeobox B13; Polyclonal Antibody; Goat anti-Homeobox B13 Antibody; homeobox B13; HOXB13; PSGD; homeo box B13; homeobox B13 antibody; HOXB13 antibody; PSGD antibody; homeo box B13 antibody; anti-HOXB13 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KKVLAKVKNSATP
Sequence Length
284
Applicable Applications for anti-HOXB13 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 28kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 30.7kDa according to NP_006352.2). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-KKVLAKVKNSATP, from the C Terminus of the protein sequence according to NP_006352.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,676 Da
NCBI Official Full Name
homeobox protein Hox-B13
NCBI Official Synonym Full Names
homeobox B13
NCBI Official Symbol
HOXB13
NCBI Official Synonym Symbols
PSGD
NCBI Protein Information
homeobox protein Hox-B13
UniProt Protein Name
Homeobox protein Hox-B13
Protein Family
UniProt Gene Name
HOXB13
UniProt Entry Name
HXB13_HUMAN

NCBI Description

This gene encodes a transcription factor that belongs to the homeobox gene family. Genes of this family are highly conserved among vertebrates and essential for vertebrate embryonic development. This gene has been implicated to play a role in fetal skin development and cutaneous regeneration. In mice, a similar gene was shown to exhibit temporal and spatial colinearity in the main body axis of the embryo, but was not expressed in the secondary axes, which suggests functions in body patterning along the axis. This gene and other HOXB genes form a gene cluster at chromosome the 17q21-22 region. [provided by RefSeq, Jul 2008]

Uniprot Description

HOXB13: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Belongs to the Abd-B homeobox family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: sequence-specific DNA binding

Biological Process: angiogenesis; epidermis development; regulation of growth; regulation of transcription, DNA-dependent; response to testosterone stimulus; response to wounding; transcription, DNA-dependent

Research Articles on HOXB13

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Product Notes

The HOXB13 hoxb13 (Catalog #AAA421172) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Homeobox B13 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Homeobox B13 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 28kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 30.7kDa according to NP_006352.2). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the HOXB13 hoxb13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KKVLAKVKNS ATP. It is sometimes possible for the material contained within the vial of "Homeobox B13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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