Rabbit anti-Human HNRPAB Polyclonal Antibody | anti-HNRPAB antibody

HNRPAB, NT (HNRNPAB, ABBP1, HNRPAB, Heterogeneous nuclear ribonucleoprotein A/B, APOBEC1-binding protein 1) (FITC)

Cat. Num. AAA6307553

• Gene Names: HNRNPAB; ABBP1; HNRPAB
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HNRPAB; Polyclonal Antibody; NT (HNRNPAB; ABBP1; Heterogeneous nuclear ribonucleoprotein A/B; APOBEC1-binding protein 1) (FITC); HNRNPAB; APOBEC1-binding protein 1; anti-HNRPAB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HNRPAB antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HNRPAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-38 amino acids from the N-terminal region of human HNRPAB.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HNRPAB Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HNRPAB antibody detected the HNRPAB protein (arrow).)

Immunohistochemistry (IHC)

(HNRPAB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HNRPAB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Fluorescent confocal image of MCF-7 cell stained with HNRPAB Antibody (N-term).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRPAB primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HNRPAB immunoreactivity is localized to Nucleus significantly.)

Related Product Information for anti-HNRPAB antibody

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs.

Product Categories/Family for anti-HNRPAB antibody

Antibodies; Nuclear Proteins

References

Jonson, L., et al. Mol. Cell Proteomics 6(5):798-811(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006). Ong, S.E., et al. Nat. Methods 1(2):119-126(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 55956919
• NCBI GeneID: 3182
• NCBI Accession #: NP_112556
• NCBI GenBank Nucleotide #: NM_031266
• UniProt Accession #: Q99729
• Molecular Weight: 36kDa
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein A/B isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein A/B
• NCBI Official Symbol: HNRNPAB
• NCBI Official Synonym Symbols: ABBP1; HNRPAB
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein A/B
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein A/B
• UniProt Gene Name: HNRNPAB
• UniProt Synonym Gene Names: ABBP1; HNRPAB; hnRNP A/B; ABBP-1
• UniProt Entry Name: ROAA_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP A/B: a single-stranded RNA binding protein with two RNA-recognition (RRM) domains. Has a high affinity for G- rich and U-rich regions of hnRNA. Also binds to APOB mRNA transcripts around the RNA editing site. Interacts with APOBEC1. Observed in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. A member of the RNA recognition motif (RRM) family. Four isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; ribonucleoprotein complex

Molecular Function: mRNA binding; RNA binding; sequence-specific DNA binding; nucleotide binding; transcription factor activity

Biological Process: positive regulation of transcription, DNA-dependent; epithelial to mesenchymal transition; negative regulation of transcription from RNA polymerase II promoter