Rabbit anti-Human HERV-FRD Polyclonal Antibody | anti-HERV-FRD antibody

HERV-FRD, NT (ERVFRD-1, ERVFRDE1, HERV-FRD_6p24.1 provirus ancestral Env polyprotein, Endogenous retrovirus group FRD member 1, Envelope polyprotein, HERV-FRD, Syncytin-2, Surface protein, Transmembrane protein) (MaxLight 750)

Cat. Num. AAA6306206

• Gene Names: ERVFRD-1; envFRD; UNQ6191; ERVFRDE1; GLLL6191; HERV-FRD; HERV-W/FRD
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HERV-FRD; Polyclonal Antibody; NT (ERVFRD-1; ERVFRDE1; HERV-FRD_6p24.1 provirus ancestral Env polyprotein; Endogenous retrovirus group FRD member 1; Envelope polyprotein; Syncytin-2; Surface protein; Transmembrane protein) (MaxLight 750); ERVFRD-1; Transmembrane protein; anti-HERV-FRD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HERV-FRD.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-HERV-FRD antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 79-107 in the N-terminal region of human HERV-FRD, UniProt Accession #P60508. Species sequence homology: monkey
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6011484. This demonstrates that MBS6011484 detected the HERV-FRD protein (arrow).)

Related Product Information for anti-HERV-FRD antibody

Human endogenous retroviruses (HERVs) make up approximately 8% of the human genome. Although most HERVs are nonfunctional, the HERV-W (ERVWE1; MIM 604659) and HERV-FRD envelope (env) proteins can induce cell-cell fusion when expressed in cells possessing appropriate receptors (Blaise et al., 2003 [PubMed 14557543]).

Product Categories/Family for anti-HERV-FRD antibody

Antibodies; Infectious Disease; HERV

References

1. Vargas, A., et al. J. Mol. Biol. 392(2):301-318(2009). 2. Chen, C.P., et al. Biol. Reprod. 79(5):815-823(2008). 3. Malassine, A., et al. Retrovirology 5, 6 (2008). 4. Mangeney, M., et al. Proc. Natl. Acad. Sci. U.S.A. 104(51):20534-20539(2007). 5. Malassine, A., et al. Placenta 28 (2-3), 185-191 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 46485773
• NCBI GeneID: 405754
• NCBI Accession #: NP_997465.1
• NCBI GenBank Nucleotide #: NM_207582.2
• UniProt Accession #: P60508
• Molecular Weight: 59,523 Da
• NCBI Official Full Name: syncytin-2 preproprotein
• NCBI Official Synonym Full Names: endogenous retrovirus group FRD member 1, envelope
• NCBI Official Symbol: ERVFRD-1
• NCBI Official Synonym Symbols: envFRD; UNQ6191; ERVFRDE1; GLLL6191; HERV-FRD; HERV-W/FRD
• NCBI Protein Information: syncytin-2
• UniProt Protein Name: Syncytin-2
• UniProt Gene Name: ERVFRD-1
• UniProt Synonym Gene Names: ERVFRDE1; SU; TM

NCBI Description

Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of a human endogenous retrovirus provirus on chromosome 6 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product plays a major role in placental development and trophoblast fusion. The protein has the characteristics of a typical retroviral envelope protein, including a cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. [provided by RefSeq, Jun 2012]

Uniprot Description

This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. The interaction with MFSD2A is apparently important for this process (PubMed:18988732).