Rabbit HNRNPUL1 Polyclonal Antibody | anti-HNRNPUL1 antibody

HNRNPUL1 Antibody

Cat. Num. AAA2524350

• Gene Names: HNRNPUL1; E1BAP5; E1B-AP5; HNRPUL1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: HNRNPUL1; Polyclonal Antibody; HNRNPUL1 Antibody; E1B-AP5; E1BAP5; FLJ12944; HNRPUL1; anti-HNRNPUL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Sequence Length: 856

• Applicable Applications for anti-HNRNPUL1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000-1:4000; IHC: 1:20-1:200; IF: 1:50-1:500
• Immunogen: Fusion protein of human HNRNPUL1
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-161714 / sc-161715 / sc-101976
• Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles

Western Blot (WB)

(Western Blot analysis of Hela cells using HNRNPUL1 Polyclonal Antibody at dilution of 1:2000)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human ovary tumor slide using HNRNPUL1 Polyclonal Antibody at dilution of 1:100)

Immunofluorescent (IF)

(Immunofluorescent analysis of HepG2 cells using HNRNPUL1 Polyclonal Antibody at dilution of 1:50)

Related Product Information for anti-HNRNPUL1 antibody

HNRNPUL1, also named as E1BAP5, HNRPUL1 and E1B-AP5, acts as a basic transcriptional regulator. It represses basic transcription driven by several virus and cellular promoters. HNRNPUL1 plays also a role in mRNA processing and transport. It is a cellular protein that interacts with the adenovirus protein E1B-55 and implicated in mRNA export. HNRNPUL1 is an hnRNP-like protein that likely mediates the interaction of NXF1 with mRNAs.(PMID:17267598). It may modulate PAN expression in complex with KSHV ORF57(PMID:22043172). HNRNPUL1 specifically bound to the Rae1–Nup98 complex independently of M protein, and this interaction was found to be RNA dependent, as it was greatly diminished in the presence of RNase.(PMID:19745842) HNRNPUL1 has 5 isoforms with calculated MW 84-95kd and 42kd(isoform 5). This antibody can recognize isoform 1-4.

NCBI and Uniprot Product Information

• NCBI GI #: 21536326
• NCBI GeneID: 11100
• NCBI Accession #: NP_008971.2
• NCBI GenBank Nucleotide #: NM_007040.4
• Molecular Weight: Claculated MW: 120 kDa; Observed MW: 95-115 kDa
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein U-like protein 1 isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein U-like 1
• NCBI Official Symbol: HNRNPUL1
• NCBI Official Synonym Symbols: E1BAP5; E1B-AP5; HNRPUL1
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein U-like protein 1; E1B 55kDa associated protein 5; E1B-55 kDa-associated protein 5; E1B-55kDa-associated protein 5; adenovirus early region 1B-associated protein 5
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein U-like protein 1
• Protein Family: Heterogeneous nuclear ribonucleoprotein U-like protein
• UniProt Gene Name: HNRNPUL1
• UniProt Synonym Gene Names: E1BAP5; HNRPUL1; E1B-AP5
• UniProt Entry Name: HNRL1_HUMAN

NCBI Description

This gene encodes a nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. This protein binds specifically to adenovirus E1B-55kDa oncoprotein. It may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by E1B-55kDa in adenovirus-infected cells. Two transcript variants encoding different isoforms have been found for this gene. Additional variants have also been found, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

E1B-AP5: a nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. Binds specifically to adenovirus E1B-55kDa oncoprotein. It may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by E1B-55kDa in adenovirus-infected cells. Four alternatively spliced isoforms have been described.

Protein type: RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleoplasm; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; enzyme binding; RNA binding

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; response to virus; gene expression