anti-Human, Rat TRAP1 Polyclonal Antibody | anti-TRAP1 antibody

Anti-TRAP1 Antibody

Cat. Num. AAA178393

• Gene Names: TRAP1; HSP75; HSP 75; HSP90L; TRAP-1
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: TRAP1; Polyclonal Antibody; Anti-TRAP1 Antibody; Heat shock protein 75 kDa; Heat shock protein 75; Heat shock protein 75 kDa mitochondrial; HSP 75; HSP 90L; HSP75; HSP90L; mitochondrial; TNF receptor associated protein 1; TNFR associated protein 1; TNFR-associated protein 1; TRAP 1; TRAP-1; Trap1; TRAP1_HUMAN; Tumor necrosis factor receptor associated protein; Tumor necrosis factor type 1 receptor associated protein; Tumor necrosis factor type 1 receptor-associated protein; TNF receptor-associated protein 1; anti-TRAP1 antibody

• Reactivity: Human, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 651

• Applicable Applications for anti-TRAP1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human TRAP1 recombinant protein (Position: A571-H704). Human TRAP1 shares 91.7% and 94% amino acid (aa) sequence identity with mouse and rat TRAP1, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- TRAP1 Picoband antibody, MBS178393, Western blottingAll lanes: Anti TRAP1 (MBS178393) at 0.5ug/mlLane 1: Rat Cardiac Muscle Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Rat Brain Tissue Lysate at 50ugLane 4: SMMC Whole Cell Lysate at 40ugLane 5: PANC Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugPredicted bind size: 80KDObserved bind size: 80KD )

Immunohistochemistry (IHC)

(Anti- TRAP1 Picoband antibody, MBS178393, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Related Product Information for anti-TRAP1 antibody

Description: Rabbit IgG polyclonal antibody for Heat shock protein 75 kDa, mitochondrial(TRAP1) detection. Tested with WB, IHC-P in Human;Rat.

Background: Heat shock protein 75 kDa, mitochondrial is a protein that in humans is encoded by the TRAP1 gene. It is mapped to 16p13.3. This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. And this protein may function in regulating cellular stress responses. In addition, it was found that TRAP1 interacted with the N-terminal half of TNFR1. Also, TRAP1 interacted with the C-terminal ends of the proteins encoded by both multiple exostoses-causing genes, EXT1 and EXT2, but not with EXTL1 or EXTL3.

References

1. "Entrez Gene: TRAP1 TNF receptor-associated protein 1". 2. Felts SJ, Owen BA, Nguyen P, Trepel J, Donner DB, Toft DO (March 2000). "The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties". J Biol Chem 275 (5): 3305-12. 3. Song HY, Dunbar JD, Zhang YX, Guo D, Donner DB (March 1995). "Identification of a protein with homology to hsp90 that binds the type 1 tumor necrosis factor receptor". J Biol Chem 270 (8): 3574-81.

NCBI and Uniprot Product Information

• NCBI GI #: 440309857
• NCBI GeneID: 10131
• NCBI Accession #: NP_001258978.1
• NCBI GenBank Nucleotide #: NM_001272049.1
• UniProt Accession #: Q12931; O43642; O75235; Q9UHL5; B4DR68; D3DUC8; F5H897
• Molecular Weight: 74,267 Da
• NCBI Official Full Name: heat shock protein 75 kDa, mitochondrial isoform 2
• NCBI Official Synonym Full Names: TNF receptor associated protein 1
• NCBI Official Symbol: TRAP1
• NCBI Official Synonym Symbols: HSP75; HSP 75; HSP90L; TRAP-1
• NCBI Protein Information: heat shock protein 75 kDa, mitochondrial
• UniProt Protein Name: Heat shock protein 75 kDa, mitochondrial
• Protein Family: TNF receptor-associated protein
• UniProt Gene Name: TRAP1
• UniProt Synonym Gene Names: HSP75; HSP 75; TRAP-1
• UniProt Entry Name: TRAP1_HUMAN

NCBI Description

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

HSP75: Chaperone that expresses an ATPase activity. Belongs to the heat shock protein 90 family.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: lipid particle; membrane; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial matrix; mitochondrion; nucleoplasm

Molecular Function: ATP binding; protein binding; protein kinase binding; tumor necrosis factor receptor binding; unfolded protein binding

Biological Process: response to stress; translational attenuation