Rabbit anti-Human HNRNPR Polyclonal Antibody | anti-HNRNPR antibody

HNRNPR Antibody (N-term)

Cat. Num. AAA9214149

• Gene Names: HNRNPR; HNRPR; hnRNP-R
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: HNRNPR; Polyclonal Antibody; HNRNPR Antibody (N-term); Heterogeneous nuclear ribonucleoprotein R; hnRNP R; HNRPR; anti-HNRNPR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This HNRNPR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-123 amino acids from the N-terminal region of human HNRNPR.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 95-123

• Applicable Applications for anti-HNRNPR antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Paraffin-Leica, Flow Cytometry (FC), ELISA (EIA)
• Application Notes: IF~~1:25; WB~~1:2000; IHC-P-Leica~~1:1000; FC~~1:25
• Function: Component of ribonucleosomes, which are complexes of at least 20 other different heterogeneous nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus.
• Cellular Location: Nucleus {ECO:0000250|UniProtKB:Q7TMK9}. Microsome {ECO:0000250|UniProtKB:Q7TMK9}. Nucleus, nucleoplasm. Cytoplasm Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. The tyrosine phosphorylated form bound to RNA is found in microsomes (By similarity). {ECO:0000250|UniProtKB:Q7TMK9, ECO:0000269|PubMed:17289661}

Immunofluorescence (IF)

(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling HNRNPR with MBS9214149 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).)

Western Blot (WB)

(HNRNPR Antibody (N-term) (MBS9214149) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HNRNPR antibody detected the HNRNPR protein (arrow).)

Western Blot (WB)

(Western blot analysis of HNRNPR (arrow) using rabbit polyclonal HNRNPR Antibody (N-term) (MBS9214149). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HNRNPR gene.)

Western Blot (WB)

(All lanes : Anti-HNRNPR Antibody (N-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: MOLT-4 whole cell lysate Lane 6: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot

(All lanes : Anti-HNRNPR Antibody (N-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: HT-29 whole cell lysate Lane 6: MOLT-4 whole cell lysate Lane 7: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71, 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human brain tissue using MBS9214149 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

Flow Cytometry (FC)

(Overlay histogram showing HeLa cells stained with MBS9214149(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9214149, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

Related Product Information for anti-HNRNPR antibody

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs and also contains a nuclear localization motif. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].

References

Hadian, K., et al. J. Biol. Chem. 284(48):33384-33391(2009)
Fukuda, A., et al. J. Biol. Chem. 284(35):23472-23480(2009)
Tonevitsky, E.A., et al. Bull. Exp. Biol. Med. 147(6):733-736(2009)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Szafranski, K., et al. Genome Biol. 8 (8), R154 (2007) :

NCBI and Uniprot Product Information

• NCBI GI #: 156151392
• NCBI GeneID: 10236
• NCBI Accession #: NP_001095867.1; NP_001095868.1; NP_005817.1
• NCBI GenBank Nucleotide #: NM_001102397.2
• UniProt Accession #: O43390; Q2L7G6; Q5TEH1; Q9BV64; S4R3J4
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein R isoform 4
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein R
• NCBI Official Symbol: HNRNPR
• NCBI Official Synonym Symbols: HNRPR; hnRNP-R
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein R
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein R
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPR
• UniProt Synonym Gene Names: HNRPR; hnRNP R
• UniProt Entry Name: HNRPR_HUMAN

NCBI Description

This gene encodes an RNA-binding protein that is a member of the spliceosome C complex, which functions in pre-mRNA processing and transport. The encoded protein also promotes transcription at the c-fos gene. Alternative splicing results in multiple transcript variants. There are pseudogenes for this gene on chromosomes 4, 11, and 10. [provided by RefSeq, Jul 2014]

Uniprot Description

hnRNP R: Component of ribonucleosomes, which are complexes of at least 20 other different heterogenious nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nucleolus; ribonucleoprotein complex

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA processing