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Western Blot (WB) (Western blot analysis of extracts of U-251MG cells, using HNRNPA3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

Rabbit HNRNPA3 Polyclonal Antibody | anti-HNRNPA3 antibody

HNRNPA3 Rabbit pAb

Gene Names
HNRNPA3; FBRNP; HNRPA3; D10S102; 2610510D13Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
HNRNPA3; Polyclonal Antibody; HNRNPA3 Rabbit pAb; 2610510D13Rik; D10S102; FBRNP; HNRPA3; anti-HNRNPA3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, pH7.3.
Applicable Applications for anti-HNRNPA3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
A synthetic Peptide of human HNRNPA3
Cellular Location
Nucleus
Positive Samples
U-251MG
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of U-251MG cells, using HNRNPA3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

Western Blot (WB) (Western blot analysis of extracts of U-251MG cells, using HNRNPA3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat ovary using HNRNPA3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat ovary using HNRNPA3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using HNRNPA3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer using HNRNPA3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using HNRNPA3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse brain using HNRNPA3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
Product Categories/Family for anti-HNRNPA3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,029 Da
NCBI Official Full Name
heterogeneous nuclear ribonucleoprotein A3
NCBI Official Synonym Full Names
heterogeneous nuclear ribonucleoprotein A3
NCBI Official Symbol
HNRNPA3
NCBI Official Synonym Symbols
FBRNP; HNRPA3; D10S102; 2610510D13Rik
NCBI Protein Information
heterogeneous nuclear ribonucleoprotein A3; hnRNP A3
UniProt Protein Name
Heterogeneous nuclear ribonucleoprotein A3
UniProt Gene Name
HNRNPA3
UniProt Synonym Gene Names
HNRPA3; hnRNP A3
UniProt Entry Name
ROA3_HUMAN

Uniprot Description

hnRNP A3: Plays a role in cytoplasmic trafficking of RNA. Binds to the cis-acting response element, A2RE. May be involved in pre-mRNA splicing. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: nucleoplasm; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression

Research Articles on HNRNPA3

Similar Products

Product Notes

The HNRNPA3 hnrnpa3 (Catalog #AAA9143605) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HNRNPA3 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HNRNPA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the HNRNPA3 hnrnpa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNRNPA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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