Rabbit hnRNP G Polyclonal Antibody | anti-hnRNPG antibody

hnRNP G (M40) Polyclonal Antibody

Cat. Num. AAA3002392

• Gene Names: RBMX; RNMX; HNRPG; HNRNPG; MRXS11; RBMXP1; RBMXRT; hnRNP-G
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: hnRNP G; Polyclonal Antibody; hnRNP G (M40) Polyclonal Antibody; RNA-binding motif protein; X chromosome; Glycoprotein p43; Heterogeneous nuclear ribonucleoprotein G; RBMX; HNRPG; RBMXP1; anti-hnRNPG antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: hnRNP G (M40) polyclonal antibody detects endogenous levels of hnRNP G protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 196

• Applicable Applications for anti-hnRNPG antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 20-60 of Human hnRNP G.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of hnRNP G (M40) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-hnRNPG antibody

hnRNP G is a glycoprotein, which was originally identified as an autoantigen from German shepherd dogs with lupus-like syndrome. The gene encoding hnRNP G is located on chromosome Xq26 and is ubiquitously expressed. It contains one RNP-consensus RNA binding domain (RBD) and is related to RBMY, which is involved in spermatogenesis, and hnRNP G-T, which is a testis specific protein. All three proteins interact with Tra2b and therefore, are involved in pre-mRNA splicing.

NCBI and Uniprot Product Information

• NCBI GI #: 258645150
• NCBI GeneID: 27316
• NCBI Accession #: NP_001158275.1
• NCBI GenBank Nucleotide #: NP_001158275.1
• UniProt Accession #: P38159
• Molecular Weight: ~ 40kDa
• NCBI Official Full Name: RNA-binding motif protein, X chromosome isoform 2
• NCBI Official Synonym Full Names: RNA binding motif protein X-linked
• NCBI Official Symbol: RBMX
• NCBI Official Synonym Symbols: RNMX; HNRPG; HNRNPG; MRXS11; RBMXP1; RBMXRT; hnRNP-G
• NCBI Protein Information: RNA-binding motif protein, X chromosome
• UniProt Protein Name: RNA-binding motif protein, X chromosome
• UniProt Gene Name: RBMX
• UniProt Synonym Gene Names: HNRPG; RBMXP1; hnRNP G
• UniProt Entry Name: RBMX_HUMAN

NCBI Description

This gene belongs to the RBMY gene family which includes candidate Y chromosome spermatogenesis genes. This gene, an active X chromosome homolog of the Y chromosome RBMY gene, is widely expressed whereas the RBMY gene evolved a male-specific function in spermatogenesis. Pseudogenes of this gene, found on chromosomes 1, 4, 9, 11, and 6, were likely derived by retrotransposition from the original gene. Alternatively spliced transcript variants encoding different isoforms have been identified. A snoRNA gene (SNORD61) is found in one of its introns. [provided by RefSeq, Sep 2009]

Uniprot Description

RBMX: RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single- stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1- beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA processing; Tumor suppressor; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: Xq26.3

Cellular Component: nucleoplasm; extracellular space; membrane; nucleus; ribonucleoprotein complex

Molecular Function: mRNA binding; protein binding; RNA binding; nucleotide binding; chromatin binding

Biological Process: positive regulation of nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; osteoblast differentiation; nuclear mRNA splicing, via spliceosome; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; membrane protein ectodomain proteolysis; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of alternative nuclear mRNA splicing, via spliceosome; protein homooligomerization