Rabbit anti-Human, Rat hnRNP C1/2 Polyclonal Antibody | anti-hnRNP antibody

hnRNP C1/2 (D271) Polyclonal Antibody

Cat. Num. AAA3002353

• Gene Names: HNRNPC; C1; C2; HNRNP; HNRPC; SNRPC
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: hnRNP C1/2; Polyclonal Antibody; hnRNP C1/2 (D271) Polyclonal Antibody; Heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2; HNRNPC; HNRPC; anti-hnRNP antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: hnRNP C1/2 (D271) polyclonal antibody detects endogenous levels of hnRNP C1/2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 306

• Applicable Applications for anti-hnRNP antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 241-290 of Human hnRNP C1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of hnRNP C1/C2 (D271) pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (20ug) Lane4:K562 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of hnRNP C1/2 (D271) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-hnRNP antibody

The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. hnRNP C1 and C2 are encoded by one gene; the two alternatively spliced transcript variants have been described.

NCBI and Uniprot Product Information

• NCBI GI #: 117190192
• NCBI GeneID: 3183
• NCBI Accession #: NP_001070910.1
• NCBI GenBank Nucleotide #: NP_001070910.1
• UniProt Accession #: P07910
• Molecular Weight: ~ 33kDa
• NCBI Official Full Name: heterogeneous nuclear ribonucleoproteins C1/C2 isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein C
• NCBI Official Symbol: HNRNPC
• NCBI Official Synonym Symbols: C1; C2; HNRNP; HNRPC; SNRPC
• NCBI Protein Information: heterogeneous nuclear ribonucleoproteins C1/C2
• UniProt Protein Name: Heterogeneous nuclear ribonucleoproteins C1/C2
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPC
• UniProt Synonym Gene Names: HNRPC; hnRNP C1/C2
• UniProt Entry Name: HNRPC_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP C1/C2: a heterogeneous nuclear ribonucleoprotein. May play a role in ribonucleosome assembly by neutralizing basic proteins such as A and B core hnRNP. Contains 1 RNA recognition motif (RRM) domain. Two alternatively spliced isoforms have been described.

Protein type: RNA-binding; Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; spliceosome; protein complex; membrane; nuclear chromatin; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; nucleosomal DNA binding; mRNA 3'-UTR binding; RNA binding; nucleotide binding; poly(U) binding

Biological Process: nuclear mRNA splicing, via spliceosome; osteoblast differentiation; RNA splicing; ATP-dependent chromatin remodeling; gene expression