Typical Testing Data/Standard Curve (for reference only)
Rabbit Lipolysaccharide binding protein ELISA Kit | LBP elisa kit
Rabbit Lipolysaccharide binding protein ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: LBP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LBP antibody and an LBP-HRP conjugate. The assay sample and buffer are incubated together with LBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LBP concentration since LBP from samples and LBP-HRP conjugate compete for the anti-LBP antibody binding site. Since the number of sites is limited, as more sites are occupied by LBP from the sample, fewer sites are left to bind LBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LBP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]
Uniprot Description
LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 20q11.23
Cellular Component: extracellular space; cell surface; extracellular region
Molecular Function: protein binding; lipopolysaccharide binding; receptor binding
Biological Process: positive regulation of tumor necrosis factor biosynthetic process; detection of molecule of bacterial origin; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of interleukin-6 production; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; positive regulation of tumor necrosis factor production; defense response to Gram-negative bacterium; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production; positive regulation of interleukin-8 production; lipopolysaccharide transport; defense response to Gram-positive bacterium; macrophage activation during immune response; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; acute-phase response; cellular defense response; innate immune response; opsonization; toll-like receptor 4 signaling pathway; positive regulation of macrophage activation
Research Articles on LBP
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Product Notes
The Rabbit LBP lbp (Catalog #AAA728345) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA728345 ELISA Kit recognizes Rabbit LBP. It is sometimes possible for the material contained within the vial of "Lipolysaccharide binding protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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