Rabbit anti-Human HMGN5 Polyclonal Antibody | anti-HMGN5 antibody

HMGN5 Antibody (N-term)

Cat. Num. AAA9203414

• Gene Names: HMGN5; NSBP1; NBP-45
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: HMGN5; Polyclonal Antibody; HMGN5 Antibody (N-term); High mobility group nucleosome-binding domain-containing protein 5; Nucleosome-binding protein 1; NSBP1; anti-HMGN5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This HMGN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-86 amino acids from the N-terminal region of human HMGN5.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.4 (varies by lot)
• Sequence Positions: 60-86
• Sequence Length: 282

• Applicable Applications for anti-HMGN5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(HMGN5 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the HMGN5 antibody detected the HMGN5 protein (arrow).)

Related Product Information for anti-HMGN5 antibody

This gene encodes a nuclear protein with similarities to
the high mobility group proteins, HMG14 and HMG17, which suggests
that this protein may function as a nucleosomal binding and
transcriptional activating protein.

References

Rochman, M., et al. Biochim. Biophys. Acta 1799 (1-2), 86-92 (2010) :
Rochman, M., et al. Mol. Cell 35(5):642-656(2009)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
King, L.M., et al. Genomics 71(2):163-173(2001)

NCBI and Uniprot Product Information

• NCBI GI #: 13540523
• NCBI GeneID: 79366
• NCBI Accession #: NP_110390.1
• NCBI GenBank Nucleotide #: NM_030763.2
• UniProt Accession #: P82970; Q5JSL1
• Molecular Weight: 31525
• NCBI Official Full Name: high mobility group nucleosome-binding domain-containing protein 5
• NCBI Official Synonym Full Names: high mobility group nucleosome binding domain 5
• NCBI Official Symbol: HMGN5
• NCBI Official Synonym Symbols: NSBP1; NBP-45
• NCBI Protein Information: high mobility group nucleosome-binding domain-containing protein 5
• UniProt Protein Name: High mobility group nucleosome-binding domain-containing protein 5
• Protein Family: High mobility group nucleosome-binding domain-containing protein
• UniProt Gene Name: HMGN5
• UniProt Synonym Gene Names: NSBP1
• UniProt Entry Name: HMGN5_HUMAN

NCBI Description

This gene encodes a nuclear protein with similarities to the high mobility group proteins, HMG14 and HMG17, which suggests that this protein may function as a nucleosomal binding and transcriptional activating protein. [provided by RefSeq, Sep 2009]

Uniprot Description

NSBP1: Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. Belongs to the HMGN family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq13.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleolus; chromatin; nucleus

Molecular Function: nucleosomal DNA binding; chromatin binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; chromatin modification