BAG1 recombinant protein

BAG1 recombinant protein

Cat. Num. AAA515337

• Gene Names: BAG1; HAP; BAG-1; RAP46
• Applications: Western Blot
• Synonyms: BAG1; BAG1 recombinant protein; RAP46; BAG1 Protein

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 3885

• Applicable Applications for BAG1 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant human BAG1 (72-end) was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for BAG1 recombinant protein

Recombinant human BAG1 (72-end) was expressed in E. coli cells using an N-terminal GST tag.

Scientific Background: BAG1 (also known as BCL2-associated athanogene) is a membrane protein rich in glutamic acid residues that binds to BCL2 and blocks apoptosis or programmed cell death (1). The BAG1-BCL2 complex enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms (2). Overexpression of BAG1 in 3T3 fibroblasts prevents apoptosis in the presence of low serum. BAG1 has also been shown to interact with activated glucocorticoid, androgen, estrogen and progesterone receptors. Binding to these receptors by BAG1 is dependent on receptor activation.

Product Categories/Family for BAG1 recombinant protein

Apoptosis Proteins; Signaling Proteins - Apoptosis Proteins

References

1.Tang, S.C. BAG-1, an anti-apoptotic tumour marker.". IUBMB Life, 2003; 53 (2): 99 -105.
2.Clemo, N.K. et al: The role of the retinoblastoma protein (Rb) in the nuclear localization of BAG-1: implications for colorectal tumour cell survival.". Biochem. Soc. Trans.2005; 33 (Pt 4): 676 -8.

NCBI and Uniprot Product Information

• NCBI GI #: 288915524
• NCBI GeneID: 573
• NCBI Accession #: NP_004314.5
• NCBI GenBank Nucleotide #: NM_004323
• UniProt Accession #: Q99933; O75315; Q14414; Q53H32; Q5VZE8; Q5VZE9; Q5VZF0; Q96TG2; Q9Y2V4
• Molecular Weight: ~66kDa
• NCBI Official Full Name: Homo sapiens BCL2-associated athanogene (BAG1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: BCL2-associated athanogene
• NCBI Official Symbol: BAG1
• NCBI Official Synonym Symbols: HAP; BAG-1; RAP46
• NCBI Protein Information: BAG family molecular chaperone regulator 1; Bcl-2-binding protein; receptor-associated protein, 46-KD; Bcl-2 associating athanogene-1 protein; glucocortoid receptor-associated protein RAP46
• UniProt Protein Name: BAG family molecular chaperone regulator 1
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG1
• UniProt Synonym Gene Names: HAP; BAG-1
• UniProt Entry Name: BAG1_HUMAN

NCBI Description

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]

Uniprot Description

Function: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. Ref.10 Ref.13 Ref.15

Subunit structure: Homodimer. Forms a heteromeric complex with HSP70/HSC70. Binds to the ATPase domain of HSP/HSC70 chaperones. Isoform 1, isoform 3 and isoform 4 but not isoform 2 interact with HSPA8/HSC70. Interacts with NR3C1. Interacts with the N-terminal region of STK19. Interacts with PPP1R15A. Interacts with BCL2 in an ATP-dependent manner. Isoform 2 does not interact with BCL2. Ref.4 Ref.10 Ref.11 Ref.12 Ref.14 Ref.15

Subcellular location: Isoform 1: Nucleus. Cytoplasm. Note: Isoform 1 localizes predominantly to the nucleus. Ref.4 Ref.11Isoform 2: Cytoplasm. Nucleus. Note: Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock. Ref.4 Ref.11Isoform 4: Cytoplasm. Nucleus. Note: Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria. Ref.4 Ref.11

Tissue specificity: Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level). Ref.11

Induction: Up-regulated during differentiation of bladder epithelial cells and down-regulated during differentiation of prostate epithelium. Ref.11

Post-translational modification: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation

Probable.

Sequence similarities: Contains 1 BAG domain.Contains 1 ubiquitin-like domain.

Sequence caution: The sequence AAD11467.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAD25045.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAD96469.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAA84624.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAH72516.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAH72518.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAH72741.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAH72742.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAW58515.1 differs from that shown. Reason: Erroneous gene model prediction.