Rabbit anti-Human HMGN1 Polyclonal Antibody | anti-HMGN1 antibody

HMGN1, NT (HMGN1, HMG14, Non-histone chromosomal protein HMG-14, High mobility group nucleosome-binding domain-containing protein 1) (Biotin)

Cat. Num. AAA6307321

• Gene Names: HMGN1; HMG14
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HMGN1; Polyclonal Antibody; NT (HMGN1; HMG14; Non-histone chromosomal protein HMG-14; High mobility group nucleosome-binding domain-containing protein 1) (Biotin); High mobility group nucleosome-binding domain-containing protein 1; anti-HMGN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-HMGN1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HMGN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HMGN1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HMGN1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HMGN1 antibody detected the HMGN1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of HMGN1 (arrow) using rabbit polyclonal HMGN1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMGN1 gene.)

Immunohistochemistry (IHC)

(HMGN1 Antibody (N-term) (AP14219a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HMGN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-HMGN1 antibody

Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.

Product Categories/Family for anti-HMGN1 antibody

Antibodies; Histones

References

Rattner, B.P., et al. Mol. Cell 34(5):620-626(2009). Cherukuri, S., et al. Mol. Biol. Cell 19(5):1816-1824(2008). Zhu, N., et al. Mol. Cell. Biol. 27(24):8859-8873(2007). Jiang, X.G., et al. Biochem. Biophys. Res. Commun. 345(4):1497-1503(2006). Hu, Y.H., et al. BMC Genomics 7, 155 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 48255933
• NCBI GeneID: 3150
• NCBI Accession #: NP_004956.5
• NCBI GenBank Nucleotide #: NM_004965.6
• UniProt Accession #: P05114; Q3KQR8
• Molecular Weight: 10,659 Da
• NCBI Official Full Name: non-histone chromosomal protein HMG-14
• NCBI Official Synonym Full Names: high mobility group nucleosome binding domain 1
• NCBI Official Symbol: HMGN1
• NCBI Official Synonym Symbols: HMG14
• NCBI Protein Information: non-histone chromosomal protein HMG-14; nonhistone chromosomal protein HMG-14; high-mobility group nucleosome binding 1; high-mobility group nucleosome binding domain 1; high-mobility group (nonhistone chromosomal) protein 14; high mobility group nucleoso
• UniProt Protein Name: Non-histone chromosomal protein HMG-14
• Protein Family: Non-histone chromosomal protein
• UniProt Gene Name: HMGN1
• UniProt Synonym Gene Names: HMG14
• UniProt Entry Name: HMGN1_HUMAN

NCBI Description

The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011]

Uniprot Description

HMGN1: a nonhistone chromosomal protein of the HMG-14/HMG-17 protein family. Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation.

Protein type: Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: 21q22.2

Cellular Component: cytoplasm; nucleus; chromatin

Molecular Function: nucleosomal DNA binding; DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; transcription-coupled nucleotide-excision repair; positive regulation of RNA elongation; pyrimidine dimer repair via nucleotide-excision repair; regulation of development, heterochronic; post-embryonic camera-type eye morphogenesis; response to UV-C; regulation of epithelial cell proliferation; response to UV-B