Rabbit anti-Human HMGCS1 Polyclonal Antibody | anti-HMGCS1 antibody

HMGCS1 Antibody

Cat. Num. AAA9404296

• Gene Names: HMGCS1; HMGCS
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: HMGCS1; Polyclonal Antibody; HMGCS1 Antibody; HMGCS; anti-HMGCS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total HMGCS1 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 2.3 mg/ml (varies by lot)
• Sequence Length: 520

• Applicable Applications for anti-HMGCS1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:2000; Immunohistochemistry: 1:50-1:200
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to a region derived from internal residues of human 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble)
• Target Name: HMGCS1
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 6%SDS-PAGE Lysates (from left to right): Human fetal liver tissue Amount of lysate: 40ug per lane Primary antibody: 1/300 dilution Secondary antibody dilution: 1/8000 Exposure time: 30 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using at dilution 1/40.)

Related Product Information for anti-HMGCS1 antibody

HMG-CoA Synthase exists as both a mitochondrial (mHMGCS) and cytoplasmic (cHMGCS) enzyme that condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA. The HMG-CoA produced by cHMGCS is transformed into mevalonate by HMG-CoA reductase, which starts isoprenoid biosynthesis. End products of the isoprenoid pathway include cholesterol, ubiquinone, dolichol, isopentenyl adenosine and farnesyl groups. mHMGCS, together with HMG-CoA Lyase, is responsible for ketone body biosynthesis. mHMGCS is expressed in liver and kidney. Fasting, cAMP and fatty acids increase the level of transcription of mHMGCS, while feeding and insulin repress it. A regulatory element within the mHMGCS promoter confers transcriptional regulation by PPAR, RXR, COUP-TF and HNF-4.

Product Categories/Family for anti-HMGCS1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 148298677
• NCBI GeneID: 3157
• NCBI Accession #: NP_001091742.1
• NCBI GenBank Nucleotide #: NM_001098272.2
• UniProt Accession #: Q01581; B2RDL8
• Molecular Weight: 57,294 Da
• NCBI Official Full Name: hydroxymethylglutaryl-CoA synthase, cytoplasmic
• NCBI Official Synonym Full Names: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble)
• NCBI Official Symbol: HMGCS1
• NCBI Official Synonym Symbols: HMGCS
• NCBI Protein Information: hydroxymethylglutaryl-CoA synthase, cytoplasmic
• UniProt Protein Name: Hydroxymethylglutaryl-CoA synthase, cytoplasmic
• Protein Family: Hydroxymethylglutaryl-CoA synthase
• UniProt Gene Name: HMGCS1
• UniProt Synonym Gene Names: HMGCS; HMG-CoA synthase
• UniProt Entry Name: HMCS1_HUMAN

Uniprot Description

HMGCS1: This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. Belongs to the HMG-CoA synthase family.

Protein type: Transferase; Carbohydrate Metabolism - butanoate; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; EC 2.3.3.10; Lipid Metabolism - synthesis and degradation of ketone bodies; Amino Acid Metabolism - valine, leucine and isoleucine degradation

Chromosomal Location of Human Ortholog: 5p14-p13

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein homodimerization activity; organic acid binding; hydroxymethylglutaryl-CoA synthase activity; drug binding; isomerase activity

Biological Process: response to drug; response to purine; response to vitamin E; response to tellurium ion; isoprenoid biosynthetic process; male gonad development; response to lipoprotein stimulus; lipid metabolic process; brain development; cellular lipid metabolic process; liver development; cholesterol biosynthetic process; response to low light intensity