Rabbit anti-Human HMGA2 Polyclonal Antibody | anti-HMGA2 antibody

HMGA2, CT (High Mobility Group AT-hook Protein 2, BABL, High Mobility Group Protein HMGI-C, HMGIC, LIPO, STQTL9) (Azide free) (HRP)

Cat. Num. AAA6307235

• Gene Names: HMGA2; BABL; LIPO; HMGIC; HMGI-C; STQTL9
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HMGA2; Polyclonal Antibody; CT (High Mobility Group AT-hook Protein 2; BABL; High Mobility Group Protein HMGI-C; HMGIC; LIPO; STQTL9) (Azide free) (HRP); anti-HMGA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HMGA2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HMGA2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 64-92 in the C-terminal region of human HMGA2, UniProt Accession #P52926. Species sequence homology: mouse
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) usingMBS6005665. This demonstrates that MBS6005665 detected the HMGA2 protein (arrow).)

Testing Data

()

Related Product Information for anti-HMGA2 antibody

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Categories/Family for anti-HMGA2 antibody

Antibodies; Transcription Factors

References

1. Mu, G., et al. Hum. Pathol. 41(4):493-502(2010). 2. Pillas, D., et al. PLoS Genet. 6 (2), E1000856 (2010). 3. Yang, T.L., et al. Ann. Hum. Genet. 74(1):11-16(2010). 4. Wei, J.J., et al. Am. J. Surg. Pathol. 34(1):18-26(2010). 5. Tay, Y., et al. Stem Cell Rev 5(4):328-333(2009). 6. Schwanbeck, R., et al. J. Biol. Chem. 275(3):1793-1801(2000). 7. Chau, K.Y., et al. Nucleic Acids Res. 23(21):4262-4266(1995) 8. Schoenmakers, E.F., et al. Nat. Genet. 10(4):436-444(1995) 9. Ashar, H.R., et al. Cell 82(1):57-65(1995) 10. Schoenmakers, E.F., et al. Genes Chromosomes Cancer 11(2):106-118(1994) 11. Manfioletti, G., et al. Nucleic Acids Res. 19(24):6793-6797(1991).

NCBI and Uniprot Product Information

• NCBI GI #: 62912482
• NCBI GeneID: 8091
• NCBI Accession #: NP_003475.1; NP_003474.1
• NCBI GenBank Nucleotide #: NM_003484.1
• UniProt Accession #: P52926; Q1M182; Q1M185; Q1M186; Q1M187; Q1M188; E7EP85; E7EWA2
• Molecular Weight: 9,742 Da
• NCBI Official Full Name: high mobility group protein HMGI-C isoform b
• NCBI Official Synonym Full Names: high mobility group AT-hook 2
• NCBI Official Symbol: HMGA2
• NCBI Official Synonym Symbols: BABL; LIPO; HMGIC; HMGI-C; STQTL9
• NCBI Protein Information: high mobility group protein HMGI-C
• UniProt Protein Name: High mobility group protein HMGI-C
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGA2
• UniProt Synonym Gene Names: HMGIC
• UniProt Entry Name: HMGA2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HMGA2: Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Interacts with E4F1. Interacts with NEK2. Belongs to the HMGA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: nucleoplasm; nuclear chromosome; nucleus

Molecular Function: protein binding; nucleosomal DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; DNA binding; AT DNA binding; DNA-dependent protein kinase activity; 5'-deoxyribose-5-phosphate lyase activity; SMAD binding; DNA bending activity; transcription factor binding

Biological Process: fat cell differentiation; establishment and/or maintenance of chromatin architecture; positive regulation of apoptosis; multicellular organismal development; positive regulation of transcription, DNA-dependent; mesodermal cell differentiation; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; base-excision repair; regulation of growth; chondrocyte differentiation; negative regulation of retroviral genome replication; mitosis; transcription, DNA-dependent; heterochromatin formation; response to virus; DNA damage response, detection of DNA damage; stem cell differentiation; DNA catabolic process, endonucleolytic; negative regulation of DNA binding; chromosome condensation; mesenchymal cell differentiation; cell division; mitotic cell cycle G2/M transition DNA damage checkpoint; chromosome breakage; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

Disease: Leiomyoma, Uterine