Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with HMGA1 Antibody (C-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).HMGA1 immunoreactivity is localized to Nucleus significantly. )
Rabbit anti-Human, Mouse HMGA1 Polyclonal Antibody | anti-HMGA1 antibody
HMGA1, CT (HMGA1, HMGIY, High mobility group protein HMG-I/HMG-Y, High mobility group AT-hook protein 1, High mobility group protein R) (AP)
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with HMGA1 Antibody (C-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).HMGA1 immunoreactivity is localized to Nucleus significantly. )
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with HMGA1 Antibody (C-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).HMGA1 immunoreactivity is localized to Nucleus significantly. )
Western Blot (WB)
(HMGA1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow).)
Western Blot (WB)
(HMGA1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow).)
Flow Cytometry (FC/FACS)
(HMGA1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(HMGA1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
HMGA1: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Belongs to the HMGA family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 6p21
Cellular Component: nucleoplasm; transcription factor complex; focal adhesion; cytosol; nucleus
Molecular Function: retinoid X receptor binding; protein binding; peroxisome proliferator activated receptor binding; enzyme binding; DNA-(apurinic or apyrimidinic site) lyase activity; ligand-dependent nuclear receptor transcription coactivator activity; DNA binding; AT DNA binding; retinoic acid receptor binding; 5'-deoxyribose-5-phosphate lyase activity; transcription factor binding
Biological Process: DNA unwinding during replication; viral reproduction; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; response to virus; DNA catabolic process, endonucleolytic; negative regulation of cell proliferation; nucleosome disassembly; regulation of transcription, DNA-dependent; negative regulation of chromatin silencing; base-excision repair; protein complex assembly; negative regulation of transcription, DNA-dependent
Research Articles on HMGA1
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Product Notes
The HMGA1 hmga1 (Catalog #AAA6307220) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HMGA1, CT (HMGA1, HMGIY, High mobility group protein HMG-I/HMG-Y, High mobility group AT-hook protein 1, High mobility group protein R) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HMGA1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HMGA1 hmga1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMGA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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