Rabbit anti-Human HLA-DQA1 Polyclonal Antibody | anti-HLA-DQA1 antibody

HLA-DQA1, ID (HLA-DQA1, HLA class II histocompatibility antigen, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1) (MaxLight 650)

Cat. Num. AAA6307107

• Gene Names: HLA-DQA1; CD; GSE; DQ-A1; CELIAC1; HLA-DQA
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HLA-DQA1; Polyclonal Antibody; ID (HLA-DQA1; HLA class II histocompatibility antigen; DQ alpha 1 chain; DC-1 alpha chain; DC-alpha; HLA-DCA; MHC class II DQA1) (MaxLight 650); MHC class II DQA1; anti-HLA-DQA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HLA-DQA1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-HLA-DQA1 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 57-84 in the central region of human HLA-DQA1, UniProt Accession #P01909; NP_002113.2.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6012253. This demonstrates that MBS6012253 detected the HLA-DQA1 protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of K562 cells using MBS6012253 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HLA-DQA1 antibody

MaxLight650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor647, DyLight649, Cy5 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Product Categories/Family for anti-HLA-DQA1 antibody

Antibodies; HLA

References

1. Donaldson, P.T., et al. J. Hepatol. 53(6):1049-1053(2010). 2. Noble, J.A., et al. Diabetes 59(11):2972-2979(2010). 3. Ferreira, R.C., et al. Nat. Genet. 42(9):777-780(2010). 4. Ovsyannikova, I.G., et al. PLoS ONE 5 (7), E11806 (2010). 5. Xun, Y.H., et al. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 23(6):430-433(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 18426975
• NCBI GeneID: 3117
• NCBI Accession #: NP_002113.2
• NCBI GenBank Nucleotide #: NM_002122.3
• UniProt Accession #: P01909; O19630; O19706; P01907; P01908; P04225; P04226; P05536; P79553; Q06751; Q29876; Q29994
• Molecular Weight: 254
• NCBI Official Full Name: HLA class II histocompatibility antigen, DQ alpha 1 chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DQ alpha 1
• NCBI Official Symbol: HLA-DQA1
• NCBI Official Synonym Symbols: CD; GSE; DQ-A1; CELIAC1; HLA-DQA
• NCBI Protein Information: HLA class II histocompatibility antigen, DQ alpha 1 chain; HLA-DCA; DC-alpha; DC-1 alpha chain; MHC HLA-DQ alpha; MHC class II DQA1; MHC class II antigen; leucocyte antigen DQA1; MHC class II HLA-DQ-alpha-1; leukocyte antigen alpha chain; MHC class II sur
• UniProt Protein Name: HLA class II histocompatibility antigen, DQ alpha 1 chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DQA1
• UniProt Entry Name: DQA1_HUMAN

Uniprot Description

HLA-DQA1 iso4:

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; membrane; lysosomal membrane; integral to plasma membrane; plasma membrane; trans-Golgi network membrane; endosome membrane; MHC class II protein complex; external side of plasma membrane

Molecular Function: MHC class II receptor activity; peptide antigen binding

Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; T cell receptor signaling pathway