Rabbit HLA-DOalpha Polyclonal Antibody | anti-HLA-DOalpha antibody

HLA-DOalpha (V111) Polyclonal Antibody

Cat. Num. AAA3003455

• Gene Names: HLA-DOA; HLADZ; HLA-DNA; HLA-DZA
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HLA-DOalpha; Polyclonal Antibody; HLA-DOalpha (V111) Polyclonal Antibody; HLA class II histocompatibility antigen; DO alpha chain; MHC DN-alpha; MHC DZ alpha; MHC class II antigen DOA; HLA-DNA; HLA-DZA; HLA-DOA; anti-HLA-DOalpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HLA-DOalpha (V111) polyclonal antibody detects endogenous levels of HLA-DOalpha protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 250

• Applicable Applications for anti-HLA-DOalpha antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 81-130 of Human HLA-DOalpha.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of HLA-DOalpha (V111) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-HLA-DOalpha antibody

Reacts with HLA-DO, a heterodimer formed by DNalpha and DObeta subunits in B cells. DNalpha and DObeta are the products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively. DO forms tight complexes with DM in the endoplasmic reticulum and is thereby sorted to lysosomal vesicles during antigen processing and presentation. It is tightly associated with DM and it is selectively expressed on antigen-presenting cells, such as B cells, dendritic cells and thymic epithelial cells. DO can enhance the efficiency of peptide loading and has been found to stabilize DM at low pH, preserving its chaperon activity. DO-DM complexes are more efficient than DM in protecting empty DR molecules. Reports describe DO as a co-chaperone of DM. This antibody has also been reported to be suitable for western blot and immunoprecipitation.

NCBI and Uniprot Product Information

• NCBI GI #: 4504401
• NCBI GeneID: 3111
• NCBI Accession #: NP_002110.1
• NCBI GenBank Nucleotide #: NP_002110.1
• UniProt Accession #: P06340
• Molecular Weight: ~ 34kDa
• NCBI Official Full Name: HLA class II histocompatibility antigen, DO alpha chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DO alpha
• NCBI Official Symbol: HLA-DOA
• NCBI Official Synonym Symbols: HLADZ; HLA-DNA; HLA-DZA
• NCBI Protein Information: HLA class II histocompatibility antigen, DO alpha chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DO alpha chain
• UniProt Gene Name: HLA-DOA
• UniProt Synonym Gene Names: HLA-DNA; HLA-DZA
• UniProt Entry Name: DOA_HUMAN

NCBI Description

HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DOA: Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: lysosomal membrane; integral to membrane; plasma membrane; endosome membrane; MHC class II protein complex

Molecular Function: MHC class II receptor activity

Biological Process: negative regulation of antigen processing and presentation of peptide antigen via MHC class II; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; regulation of T cell differentiation; signal transduction