Rabbit anti-Human HLA Class 1 Antigen B Polyclonal Antibody | anti-HLAB antibody

HLA Class 1 Antigen B, NT (HLA Cass I Histocompatibility Antigen B-27 alpha Chain, HLA B Class I, HLA-B, HLAB, HLA-B27, HLA-B73, AS, MGC111087, MHC Class I Antigen B*27, SPDA1) (FITC)

Cat. Num. AAA6480912

• Gene Names: HLA-B; AS; HLAB; SPDA1
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HLA Class 1 Antigen B; Polyclonal Antibody; NT (HLA Cass I Histocompatibility Antigen B-27 alpha Chain; HLA B Class I; HLA-B; HLAB; HLA-B27; HLA-B73; AS; MGC111087; MHC Class I Antigen B*27; SPDA1) (FITC); Major Histocompatibility Complex; Class I; B; anti-HLAB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HLA Class 1 Antigen B.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HLAB antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 62-90 in the N-terminal region of human HLA-B, UniProt Accession #P03989
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of A2058 cell line lysates (35ug/lane) using H6098-40T. HLA-B (arrow) was detected using H6098-40T.)

Western Blot (WB)

(Western Blot analysis of A375 cell line lysates (35ug/lane) using H6098-40T. HLA-B (arrow) was detected using H6098-40T.)

Western Blot (WB)

(Western Blot analysis of HLA-B (arrow) in 293 cell lysates (2ug/lane) using H6098-40T Lane 1: nontransfected Lane 2: transiently transfected with the HLA-B gene.)

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed and paraffin-embedded human hepatocarcinoma using H6098-40T and peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of H6098-40T for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of human peripheral blood lymphocytes cells using H6098-40T (green) compared to an isotype control of rabbit IgG (blue). H6098-40T was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Product Categories/Family for anti-HLAB antibody

Antibodies; HLA

References

1. Higgins, C.M., et.al., Ann. Rheum. Dis. 51(7), 855-862 (1992). 2. Guild, B.C.et.al., J. Biol. Chem. 259(14), 9235-9240 (1984). 3. Orr, H.T., et.alt., Biochemistry 18(25), 5711-5720 (1979).

NCBI and Uniprot Product Information

• NCBI GI #: 17986001
• NCBI GeneID: 3106
• NCBI Accession #: NP_005505.2
• NCBI GenBank Nucleotide #: NM_005514.6
• UniProt Accession #: P03989; Q29638; Q29681; Q29854; Q29861; Q31613; Q5SRJ2; Q9GIX1; Q9TP95; P01889; P10319; P18463; P18464; P18465; P30460; P30461; P30462; P30464
• Molecular Weight: 40,460 Da
• NCBI Official Full Name: major histocompatibility complex, class I, B
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, B
• NCBI Official Symbol: HLA-B
• NCBI Official Synonym Symbols: AS; HLAB; SPDA1
• NCBI Protein Information: major histocompatibility complex, class I, B; HLA class I histocompatibility antigen, B alpha chain; MHC Class I HLA heavy chain; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen GN00104; MHC class I antigen H
• UniProt Protein Name: HLA class I histocompatibility antigen, B-27 alpha chain
• UniProt Gene Name: HLA-B
• UniProt Synonym Gene Names: HLAB

NCBI Description

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the presentation of foreign antigens to the immune system.