Rabbit HLA-A Polyclonal Antibody | anti-HLA-A antibody

Anti-HLA-A Antibody

Cat. Num. AAA2401925

• Gene Names: HLA-A; HLAA
• Reactivity: Human; Predicted: Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: HLA-A; Polyclonal Antibody; Anti-HLA-A Antibody; Mouse Monoclonal (IgG2b) to Human HLA-A; Leukocyte antigen class I-A; HLAA; HLA class I histocompatibility antigen; A-1 alpha chain; anti-HLA-A antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human HLA-A
• Purity/Purification: Affinity Purified
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
• Sequence Length: 365

• Applicable Applications for anti-HLA-A antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 1:50-1:200; IHC: 1:50-1:200; IHC-P: 1:100; WB: 1:500-1:2000
• Immunogen: Recombinant protein of human HLA-A
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using HLA-A antibody. Blue: DAPI for nuclear staining)

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HLA-A antibody.)

Western Blot (WB)

(Western blot analysis of extracts of various cell lines.)

Related Product Information for anti-HLA-A antibody

HLA-A belongs to the HLA class I heavy chain paralogues. This class Imolecule is a heterodimer consisting of a heavy chain and a light chain(beta-2 microglobulin). the heavy chain is anchored in the membrane.Class I molecules play a central role in the immune system by presentingpeptides derived from the endoplasmic reticulum lumen. they areexpressed in nearly all cells. the heavy chain is approximately 45 kDa and its gene contains 8 exons.

NCBI and Uniprot Product Information

• NCBI GI #: 24797067
• NCBI GeneID: 3105
• NCBI Accession #: NP_002107.3
• NCBI GenBank Nucleotide #: NM_002116.7
• UniProt Accession #: P04439; O19546; O19756; Q65A82; Q9GJE6; Q9GJE7; Q9GJE8; Q9MYE6; Q9MYG4; Q9TPR8; P01891; P01892; P05534; P10314; P10316; P13746; P16188; P16189; P16190
• Molecular Weight: 40,841 Da
• NCBI Official Full Name: HLA class I histocompatibility antigen, A-1 alpha chain A*03:01:0:01
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, A
• NCBI Official Symbol: HLA-A
• NCBI Official Synonym Symbols: HLAA
• NCBI Protein Information: HLA class I histocompatibility antigen, A-1 alpha chain; LOW QUALITY PROTEIN: HLA class I histocompatibility antigen, A-1 alpha chain
• UniProt Protein Name: HLA class I histocompatibility antigen, A-3 alpha chain
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-A
• UniProt Synonym Gene Names: HLAA

NCBI Description

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the presentation of foreign antigens to the immune system.