Rabbit anti-Human hKv11.1 Polyclonal Antibody | anti-hKv11.1 antibody

Anti-hKv11.1 (HERG) Antibody

Cat. Num. AAA4159213

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST, and then anti-HERG antibody was Affinity Purified on immobilized HERG-GST
• Synonyms: hKv11.1; Polyclonal Antibody; Anti-hKv11.1 (HERG) Antibody; hK v11-1; anti-hKv11.1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody recognizes the HERG1b splice variant but not splice variants HERG1-3 and HERG-4
• Purity/Purification: The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST, and then anti-HERG antibody was Affinity Purified on immobilized HERG-GST
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-hKv11.1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: HEK 293-HERG transfected cells (1:200-1:400); IP: 2-5 ug.
• Immunogen: GST fusion protein with sequence DSLSQ VSQFM ACEEL PPGAP ELPQE GPTRR LSLPG QLGAL TSQPL HRHGS DPGS, corresponding to residues 1106-1159 of human erg (HERG) (Accession Q12809).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Testing Data

Related Product Information for anti-hKv11.1 antibody

Voltage gated K+ channel subfamily H member 2, KCNH2, ether-a-go-go-related channel The KV11.1 (HERG) channel is a member of the ether-a-go-go (EAG) subfamily of voltage-dependent K+ channels that includes the related proteins KV11.2 and KV11.3 (erg2 and erg3). KV11.1 possess the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains and intracellular N and C termini. The KV11.1 current is characterized by strong inward rectification with slow activation and very rapid inactivation kinetics. The channel is expressed in the brain and heart (where it underlies the IKr current) and has a central role in mediating repolarization of action potentials.1, 2 Mutations in the KV11.1 channel cause inherited long QT syndrome (LQTS) or abnormalities in the repolarization of the heart that are associated with life-threatening arrhythmias and sudden death. All the identified KV11.1 mutations produce loss of function of the channel via several cellular mechanisms ranging from alterations of gating properties, alterations of channel permeability/selectivity and alterations in intracellular channel trafficking that decreases the number of channels that reach the cell membrane.1, 2 Lately drug-induced forms of LQTS have been reported for a wide range of non-cardiac drugs including antihistamines, psychoactive agents and antimicrobials. All these drugs potently block the KV11.1 channel as an unintended side effect, prompting regulatory drug agencies to issue recommendations for the testing of new drugs for their potential KV11.1 blocking effect. In addition, KV11.1 expression was found to be upregulated in several tumor cell lines of different histogenesis suggesting that it confers the cells some advantage in cell proliferation. Indeed, in several studies it has been shown that inhibition of the KV11.1 current leads to a decrease in tumor cell proliferation.3